Human OATP-E cDNA Clone in Mammalian Expression Vector
This is a Organic Anion Transporter E plasmid from OriGene - 2 times cited - with cDNA insert cloned into Mammalian Expression VectorpCMV6-XL4. Insert length: 2700 bp. Transient expression. Suitable for PExp.
Bacterial selection: Ampicillin.
1 image
(2 references)
Catalog No. ABIN3380070
Supplier Product No.: sc108172
$655.38
Plus shipping costs $50.00
10 μg
Shipping to:
United States
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Quick Overview for Human OATP-E cDNA Clone in Mammalian Expression Vector (ABIN3380070)
Gene
SLCO4A1 (OATP-E)
(Organic Anion Transporter E (OATP-E))
Application
Protein Expression (PExp)
Insert
cDNA
Vector
Mammalian Expression Vector
Vector Backbone
pCMV6-XL4
Promoter
Enhanced CMV Promoter, T7 Promoter
Bacterial Resistance
Ampicillin
Expression Type
Transient
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Species
- Human
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Supplier Product No.
- sc108172
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Supplier
- OriGene
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Purpose
- Untagged full-length cDNA clone from Human SLCO4A1 is ideal for over-expression of native protein for functional studies.
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Specificity
- Restriction Site: NotI-NotI
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Characteristics
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- These cDNA clones are isolated from full-length cDNA libraries and usually contain the coding sequence as well as the untranslated regions (UTRs) of the mRNA transcript appropriate to the library from which they were isolated.
- These cDNA clones are ideal for over-expression of native proteins for functional studies. Provided as 10 μg transfection-ready plasmids.
- Every lot of primer is tested to provide clean sequencing of cDNA clones.
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Purification
- The DNAs were purified using PowerPrep HP Plasmid isolation kits for transfection ready plasmids.
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Components
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- The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA.
- The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
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Insert Length
- 2700 bp
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Sequencing Primer
- VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3', XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Storage
- RT,-20 °C
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Storage Comment
- The lyophilized plasmid is stable for up to one year when stored at ambient temperature. Following dissolution in 100 μL dH2O, store at -20 °C. Lyophilized primers are stable for up to one year when stored at ambient temperature. Following dissolution in 10 μL dH2O, store at -20 °C.
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Expiry Date
- 12 months
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: "Role of OATP transporters in steroid uptake by prostate cancer cells in vivo." in: Prostate cancer and prostatic diseases, (2016) (PubMed).
: "Mechanisms of pH-gradient driven transport mediated by organic anion polypeptide transporters." in: American journal of physiology. Cell physiology, Vol. 296, Issue 3, pp. C570-82, (2009) (PubMed).
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- SLCO4A1 (OATP-E) (Organic Anion Transporter E (OATP-E))
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Alternative Name
- SLCO4A1
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Background
- Mediates the Na(+)-independent transport of organic anions such as the thyroid hormones T3 (triiodo-L-thyronine), T4 (thyroxine) and rT3, and of estrone-3-sulfate and taurocholate. [UniProtKB/Swiss-Prot Function]
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NCBI Accession
- NM_016354, NP_057438
Target
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