Human TFDP1 cDNA Clone in Mammalian Expression Vector
This is a Transcription Factor Dp-1 plasmid from OriGene - 2 times cited - with cDNA insert cloned into Mammalian Expression VectorpCMV6-XL4. Insert length: 2650 bp. Transient expression. Suitable for PExp.
Bacterial selection: Ampicillin.
1 image
(2 references)
Catalog No. ABIN3380310
Supplier Product No.: sc127935
$452.43
Plus shipping costs $50.00
10 μg
Shipping to:
United States
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Quick Overview for Human TFDP1 cDNA Clone in Mammalian Expression Vector (ABIN3380310)
Gene
DP1 (TFDP1)
(Transcription Factor Dp-1 (TFDP1))
Application
Protein Expression (PExp)
Insert
cDNA
Vector
Mammalian Expression Vector
Vector Backbone
pCMV6-XL4
Promoter
Enhanced CMV Promoter, T7 Promoter
Bacterial Resistance
Ampicillin
Expression Type
Transient
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Species
- Human
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Supplier Product No.
- sc127935
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Supplier
- OriGene
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Purpose
- Untagged full-length cDNA clone from Human TFDP1 is ideal for over-expression of native protein for functional studies.
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Specificity
- Restriction Site: NotI-NotI
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Characteristics
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- These cDNA clones are isolated from full-length cDNA libraries and usually contain the coding sequence as well as the untranslated regions (UTRs) of the mRNA transcript appropriate to the library from which they were isolated.
- These cDNA clones are ideal for over-expression of native proteins for functional studies. Provided as 10 μg transfection-ready plasmids.
- Every lot of primer is tested to provide clean sequencing of cDNA clones.
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Purification
- The DNAs were purified using PowerPrep HP Plasmid isolation kits for transfection ready plasmids.
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Components
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- The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA.
- The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
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Insert Length
- 2650 bp
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Sequencing Primer
- VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3', XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Storage
- RT,-20 °C
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Storage Comment
- The lyophilized plasmid is stable for up to one year when stored at ambient temperature. Following dissolution in 100 μL dH2O, store at -20 °C. Lyophilized primers are stable for up to one year when stored at ambient temperature. Following dissolution in 10 μL dH2O, store at -20 °C.
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Expiry Date
- 12 months
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: "ZBTB32 is an early repressor of the CIITA and MHC class II gene expression during B cell differentiation to plasma cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 189, Issue 5, pp. 2393-403, (2012) (PubMed).
: "Cyclin-dependent kinase-mediated phosphorylation plays a critical role in the oncogenic functions of PELP1." in: Cancer research, Vol. 70, Issue 18, pp. 7166-75, (2010) (PubMed).
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: "ZBTB32 is an early repressor of the CIITA and MHC class II gene expression during B cell differentiation to plasma cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 189, Issue 5, pp. 2393-403, (2012) (PubMed).
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- DP1 (TFDP1) (Transcription Factor Dp-1 (TFDP1))
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Alternative Name
- TFDP1
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Background
- This gene encodes a member of a family of transcription factors that heterodimerize with E2F proteins to enhance their DNA-binding activity and promote transcription from E2F target genes. The encoded protein functions as part of this complex to control the transcriptional activity of numerous genes involved in cell cycle progression from G1 to S phase. Alternative splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 1, 15, and X.[provided by RefSeq, Jan 2009].Transcript Variant: This variant (1) represents the longer transcript.
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NCBI Accession
- NM_007111, NP_009042
Target
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