Human TNFRSF13B cDNA Clone in Mammalian Expression Vector
This is a Tumor Necrosis Factor Receptor Superfamily, Member 13B plasmid from OriGene - one-time cited - with cDNA insert cloned into Mammalian Expression VectorpCMV6-XL4. Insert length: 1500 bp. Transient expression. Suitable for PExp.
Bacterial selection: Ampicillin.
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Catalog No. ABIN3380405
Supplier Product No.: sc115358
$297.00
Plus shipping costs $50.00
10 μg
Shipping to:
United States
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Quick Overview for Human TNFRSF13B cDNA Clone in Mammalian Expression Vector (ABIN3380405)
Gene
TACI (TNFRSF13B)
(Tumor Necrosis Factor Receptor Superfamily, Member 13B (TNFRSF13B))
Application
Protein Expression (PExp)
Insert
cDNA
Vector
Mammalian Expression Vector
Vector Backbone
pCMV6-XL4
Promoter
Enhanced CMV Promoter, T7 Promoter
Bacterial Resistance
Ampicillin
Expression Type
Transient
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Species
- Human
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Supplier Product No.
- sc115358
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Supplier
- OriGene
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Purpose
- Untagged full-length cDNA clone from Human TNFRSF13B is ideal for over-expression of native protein for functional studies.
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Specificity
- Restriction Site: NotI-NotI
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Characteristics
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- These cDNA clones are isolated from full-length cDNA libraries and usually contain the coding sequence as well as the untranslated regions (UTRs) of the mRNA transcript appropriate to the library from which they were isolated.
- These cDNA clones are ideal for over-expression of native proteins for functional studies. Provided as 10 μg transfection-ready plasmids.
- Every lot of primer is tested to provide clean sequencing of cDNA clones.
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Purification
- The DNAs were purified using PowerPrep HP Plasmid isolation kits for transfection ready plasmids.
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Components
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- The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA.
- The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
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Insert Length
- 1500 bp
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Sequencing Primer
- VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3', XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Storage
- RT,-20 °C
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Storage Comment
- The lyophilized plasmid is stable for up to one year when stored at ambient temperature. Following dissolution in 100 μL dH2O, store at -20 °C. Lyophilized primers are stable for up to one year when stored at ambient temperature. Following dissolution in 10 μL dH2O, store at -20 °C.
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Expiry Date
- 12 months
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: "The immunoregulator soluble TACI is released by ADAM10 and reflects B cell activation in autoimmunity." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 194, Issue 2, pp. 542-52, (2015) (PubMed).
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: "The immunoregulator soluble TACI is released by ADAM10 and reflects B cell activation in autoimmunity." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 194, Issue 2, pp. 542-52, (2015) (PubMed).
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- TACI (TNFRSF13B) (Tumor Necrosis Factor Receptor Superfamily, Member 13B (TNFRSF13B))
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Alternative Name
- TNFRSF13B
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Background
- The protein encoded by this gene is a lymphocyte-specific member of the tumor necrosis factor (TNF) receptor superfamily. It interacts with calcium-modulator and cyclophilin ligand (CAML). The protein induces activation of the transcription factors NFAT, AP1, and NF-kappa-B and plays a crucial role in humoral immunity by interacting with a TNF ligand. This gene is located within the Smith-Magenis syndrome region on chromosome 17. [provided by RefSeq, Jul 2008].
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NCBI Accession
- NM_012452, NP_036584
Target
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