Human VTI1B cDNA Clone in Mammalian Expression Vector
This is a Vesicle Transport through Interaction with T-SNAREs Homolog 1B plasmid from OriGene - one-time cited - with cDNA insert cloned into Mammalian Expression VectorpCMV6-XL4. Insert length: 5550 bp. Transient expression. Suitable for PExp.
Bacterial selection: Ampicillin.
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(1 reference)
Catalog No. ABIN3380631
Supplier Product No.: sc116155
$297.00
Plus shipping costs $50.00
10 μg
Shipping to:
United States
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Quick Overview for Human VTI1B cDNA Clone in Mammalian Expression Vector (ABIN3380631)
Gene
VTI1B
(Vesicle Transport through Interaction with T-SNAREs Homolog 1B (VTI1B))
Application
Protein Expression (PExp)
Insert
cDNA
Vector
Mammalian Expression Vector
Vector Backbone
pCMV6-XL4
Promoter
Enhanced CMV Promoter, T7 Promoter
Bacterial Resistance
Ampicillin
Expression Type
Transient
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Species
- Human
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Supplier Product No.
- sc116155
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Supplier
- OriGene
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Purpose
- Untagged full-length cDNA clone from Human VTI1B is ideal for over-expression of native protein for functional studies.
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Specificity
- Restriction Site: NotI-NotI
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Characteristics
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- These cDNA clones are isolated from full-length cDNA libraries and usually contain the coding sequence as well as the untranslated regions (UTRs) of the mRNA transcript appropriate to the library from which they were isolated.
- These cDNA clones are ideal for over-expression of native proteins for functional studies. Provided as 10 μg transfection-ready plasmids.
- Every lot of primer is tested to provide clean sequencing of cDNA clones.
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Purification
- The DNAs were purified using PowerPrep HP Plasmid isolation kits for transfection ready plasmids.
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Components
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- The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA.
- The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
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Insert Length
- 5550 bp
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Sequencing Primer
- VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3', XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Storage
- RT,-20 °C
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Storage Comment
- The lyophilized plasmid is stable for up to one year when stored at ambient temperature. Following dissolution in 100 μL dH2O, store at -20 °C. Lyophilized primers are stable for up to one year when stored at ambient temperature. Following dissolution in 10 μL dH2O, store at -20 °C.
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Expiry Date
- 12 months
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: "Syntaxin 6 and CAL mediate the degradation of the cystic fibrosis transmembrane conductance regulator." in: Molecular biology of the cell, Vol. 21, Issue 7, pp. 1178-87, (2010) (PubMed).
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- VTI1B (Vesicle Transport through Interaction with T-SNAREs Homolog 1B (VTI1B))
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Alternative Name
- VTI1B
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Background
- V-SNARE that mediates vesicle transport pathways through interactions with t-SNAREs on the target membrane. These interactions are proposed to mediate aspects of the specificity of vesicle trafficking and to promote fusion of the lipid bilayers. May be concerned with increased secretion of cytokines associated with cellular senescence. [UniProtKB/Swiss-Prot Function]
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NCBI Accession
- NM_006370, NP_006361
Target
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