Human ZBTB10 cDNA Clone in Mammalian Expression Vector
Quick Overview for Human ZBTB10 cDNA Clone in Mammalian Expression Vector (ABIN3380700)
Gene
Application
Insert
Vector
Vector Backbone
Promoter
Bacterial Resistance
Expression Type
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Species
- Human
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Supplier Product No.
- sc128217
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Supplier
- OriGene
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Purpose
- Untagged full-length cDNA clone from Human ZBTB10 is ideal for over-expression of native protein for functional studies.
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Specificity
- Restriction Site: NotI-NotI
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Characteristics
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- These cDNA clones are isolated from full-length cDNA libraries and usually contain the coding sequence as well as the untranslated regions (UTRs) of the mRNA transcript appropriate to the library from which they were isolated.
- These cDNA clones are ideal for over-expression of native proteins for functional studies. Provided as 10 μg transfection-ready plasmids.
- Every lot of primer is tested to provide clean sequencing of cDNA clones.
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Purification
- The DNAs were purified using PowerPrep HP Plasmid isolation kits for transfection ready plasmids.
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Components
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- The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA.
- The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
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Insert Length
- 510 bp
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Sequencing Primer
- VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3', XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Storage
- RT,-20 °C
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Storage Comment
- The lyophilized plasmid is stable for up to one year when stored at ambient temperature. Following dissolution in 100 μL dH2O, store at -20 °C. Lyophilized primers are stable for up to one year when stored at ambient temperature. Following dissolution in 10 μL dH2O, store at -20 °C.
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Expiry Date
- 12 months
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: "Metformin inhibits pancreatic cancer cell and tumor growth and downregulates Sp transcription factors." in: Carcinogenesis, Vol. 34, Issue 12, pp. 2870-9, (2013) (PubMed).
: "Methyl 2-cyano-3,12-dioxooleana-1,9-dien-28-oate decreases specificity protein transcription factors and inhibits pancreatic tumor growth: role of microRNA-27a." in: Molecular pharmacology, Vol. 78, Issue 2, pp. 226-36, (2010) (PubMed).
: "PCPH/ENTPD5 expression enhances the invasiveness of human prostate cancer cells by a protein kinase C delta-dependent mechanism." in: Cancer research, Vol. 67, Issue 22, pp. 10859-68, (2007) (PubMed).
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- ZBTB10 (Zinc Finger and BTB Domain-Containing Protein 10 (ZBTB10))
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Alternative Name
- ZBTB10
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Background
- May be involved in transcriptional regulation. [UniProtKB/Swiss-Prot Function]Transcript Variant: This variant (2) lacks a segment in an internal exon, but maintains the reading frame, compared to variant 1. The encoded isoform (b) is shorter than isoform a.
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NCBI Accession
- NM_023929, NP_076418
Target
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