Human ACTA1 cDNA Clone in Mammalian Expression Vector
This is a Actin, alpha 1, Skeletal Muscle plasmid from OriGene - one-time cited - with cDNA insert cloned into Mammalian Expression VectorpCMV6-XL4. Insert length: 1600 bp. Transient expression. Suitable for PExp.
Bacterial selection: Ampicillin.
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(1 reference)
Catalog No. ABIN3380854
Supplier Product No.: sc125333
$679.14
Plus shipping costs $50.00
10 μg
Shipping to:
United States
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Quick Overview for Human ACTA1 cDNA Clone in Mammalian Expression Vector (ABIN3380854)
Gene
Actin (ACTA1)
(Actin, alpha 1, Skeletal Muscle (ACTA1))
Application
Protein Expression (PExp)
Insert
cDNA
Vector
Mammalian Expression Vector
Vector Backbone
pCMV6-XL4
Promoter
Enhanced CMV Promoter, T7 Promoter
Bacterial Resistance
Ampicillin
Expression Type
Transient
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Species
- Human
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Supplier Product No.
- sc125333
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Supplier
- OriGene
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Purpose
- Untagged full-length cDNA clone from Human ACTA1 is ideal for over-expression of native protein for functional studies.
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Characteristics
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- These cDNA clones are isolated from full-length cDNA libraries and usually contain the coding sequence as well as the untranslated regions (UTRs) of the mRNA transcript appropriate to the library from which they were isolated.
- These cDNA clones are ideal for over-expression of native proteins for functional studies. Provided as 10 μg transfection-ready plasmids.
- Every lot of primer is tested to provide clean sequencing of cDNA clones.
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Purification
- The DNAs were purified using PowerPrep HP Plasmid isolation kits for transfection ready plasmids.
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Components
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- The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA.
- The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
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Insert Length
- 1600 bp
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Sequencing Primer
- VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3', XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Storage
- RT,-20 °C
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Storage Comment
- The lyophilized plasmid is stable for up to one year when stored at ambient temperature. Following dissolution in 100 μL dH2O, store at -20 °C. Lyophilized primers are stable for up to one year when stored at ambient temperature. Following dissolution in 10 μL dH2O, store at -20 °C.
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Expiry Date
- 12 months
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: "Therapeutic impact of systemic AAV-mediated RNA interference in a mouse model of myotonic dystrophy." in: Human molecular genetics, Vol. 24, Issue 17, pp. 4971-83, (2015) (PubMed).
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: "Therapeutic impact of systemic AAV-mediated RNA interference in a mouse model of myotonic dystrophy." in: Human molecular genetics, Vol. 24, Issue 17, pp. 4971-83, (2015) (PubMed).
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- Actin (ACTA1) (Actin, alpha 1, Skeletal Muscle (ACTA1))
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Alternative Name
- ACTA1
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Background
- The product encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Mutations in this gene cause nemaline myopathy type 3, congenital myopathy with excess of thin myofilaments, congenital myopathy with cores, and congenital myopathy with fiber-type disproportion, diseases that lead to muscle fiber defects. [provided by RefSeq, Jul 2008].
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NCBI Accession
- NM_001100, NP_001091
Target
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