Human APTX cDNA Clone in Mammalian Expression Vector
This is a Aprataxin plasmid from OriGene - one-time cited - with cDNA insert cloned into Mammalian Expression VectorpCMV6-XL4. Insert length: not_set. Transient expression. Suitable for PExp.
Bacterial selection: Ampicillin.
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Catalog No. ABIN3380901
Supplier Product No.: sc312880
$445.50
Plus shipping costs $50.00
10 μg
Shipping to:
United States
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Quick Overview for Human APTX cDNA Clone in Mammalian Expression Vector (ABIN3380901)
Gene
Aprataxin (APTX)
Application
Protein Expression (PExp)
Insert
cDNA
Vector
Mammalian Expression Vector
Vector Backbone
pCMV6-XL4
Promoter
Enhanced CMV Promoter, T7 Promoter
Bacterial Resistance
Ampicillin
Expression Type
Transient
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Species
- Human
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Supplier Product No.
- sc312880
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Supplier
- OriGene
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Purpose
- Untagged full-length cDNA clone from Human APTX is ideal for over-expression of native protein for functional studies.
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Characteristics
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- These cDNA clones are isolated from full-length cDNA libraries and usually contain the coding sequence as well as the untranslated regions (UTRs) of the mRNA transcript appropriate to the library from which they were isolated.
- These cDNA clones are ideal for over-expression of native proteins for functional studies. Provided as 10 μg transfection-ready plasmids.
- Every lot of primer is tested to provide clean sequencing of cDNA clones.
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Purification
- The DNAs were purified using PowerPrep HP Plasmid isolation kits for transfection ready plasmids.
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Components
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- The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA.
- The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
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Sequencing Primer
- VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3', XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Storage
- RT,-20 °C
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Storage Comment
- The lyophilized plasmid is stable for up to one year when stored at ambient temperature. Following dissolution in 100 μL dH2O, store at -20 °C. Lyophilized primers are stable for up to one year when stored at ambient temperature. Following dissolution in 10 μL dH2O, store at -20 °C.
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Expiry Date
- 12 months
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: "Aprataxin localizes to mitochondria and preserves mitochondrial function." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 108, Issue 18, pp. 7437-42, (2011) (PubMed).
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- Aprataxin (APTX)
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Alternative Name
- APTX
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Background
- This gene encodes a member of the histidine triad (HIT) superfamily. The encoded protein may play a role in single-stranded DNA repair through its nucleotide-binding activity and its diadenosine polyphosphate hydrolase activity. Mutations in this gene have been associated with ataxia-ocular apraxia. Alternatively spliced transcript variants have been identified for this gene.[provided by RefSeq, Aug 2010].Transcript Variant: This variant (2) has multiple differences compared to variant 1. These differences result in translation initiation at an upstream ATG and an isoform (b) with a longer N-terminus and a shorter C-terminus compared to isoform a.
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NCBI Accession
- NM_175069, NP_778239
Target
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