Human ATP7A cDNA Clone in Mammalian Expression Vector
This is a ATPase, Cu++ Transporting, alpha Polypeptide plasmid from OriGene - one-time cited - with cDNA insert cloned into Mammalian Expression VectorpCMV6-XL4. Insert length: 5600 bp. Transient expression. Suitable for PExp.
Bacterial selection: Ampicillin.
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(1 reference)
Catalog No. ABIN3380923
Supplier Product No.: sc308719
$1,906.74
Plus shipping costs $50.00
10 μg
Shipping to:
United States
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Quick Overview for Human ATP7A cDNA Clone in Mammalian Expression Vector (ABIN3380923)
Gene
ATP7A
(ATPase, Cu++ Transporting, alpha Polypeptide (ATP7A))
Application
Protein Expression (PExp)
Insert
cDNA
Vector
Mammalian Expression Vector
Vector Backbone
pCMV6-XL4
Promoter
Enhanced CMV Promoter, T7 Promoter
Bacterial Resistance
Ampicillin
Expression Type
Transient
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Species
- Human
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Supplier Product No.
- sc308719
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Supplier
- OriGene
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Purpose
- Untagged full-length cDNA clone from Human ATP7A is ideal for over-expression of native protein for functional studies.
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Characteristics
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- These cDNA clones are isolated from full-length cDNA libraries and usually contain the coding sequence as well as the untranslated regions (UTRs) of the mRNA transcript appropriate to the library from which they were isolated.
- These cDNA clones are ideal for over-expression of native proteins for functional studies. Provided as 10 μg transfection-ready plasmids.
- Every lot of primer is tested to provide clean sequencing of cDNA clones.
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Purification
- The DNAs were purified using PowerPrep HP Plasmid isolation kits for transfection ready plasmids.
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Components
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- The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA.
- The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
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Insert Length
- 5600 bp
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Sequencing Primer
- VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3', XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Storage
- RT,-20 °C
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Storage Comment
- The lyophilized plasmid is stable for up to one year when stored at ambient temperature. Following dissolution in 100 μL dH2O, store at -20 °C. Lyophilized primers are stable for up to one year when stored at ambient temperature. Following dissolution in 10 μL dH2O, store at -20 °C.
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Expiry Date
- 12 months
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: "Biased agonism as a novel strategy to harness the proresolving properties of melanocortin receptors without eliciting melanogenic effects." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 194, Issue 7, pp. 3381-8, (2015) (PubMed).
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- ATP7A (ATPase, Cu++ Transporting, alpha Polypeptide (ATP7A))
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Alternative Name
- ATP7A
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Background
- This gene encodes a transmembrane protein that functions in copper transport across membranes. This protein is localized to the trans Golgi network, where it is predicted to supply copper to copper-dependent enzymes in the secretory pathway. It relocalizes to the plasma membrane under conditions of elevated extracellular copper, and functions in the efflux of copper from cells. Mutations in this gene are associated with Menkes disease, X-linked distal spinal muscular atrophy, and occipital horn syndrome. Alternatively-spliced transcript variants have been observed. [provided by RefSeq, Aug 2013].Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (1).
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NCBI Accession
- NM_000052, NP_000043
Target
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