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Human CACNG7 cDNA Clone in Mammalian Expression Vector

This is a Calcium Channel, Voltage-Dependent, gamma Subunit 7 plasmid from OriGene - 2 times cited - with cDNA insert cloned into Mammalian Expression VectorpCMV6-XL4. Insert length: 1200 bp. Transient expression. Suitable for PExp. Bacterial selection: Ampicillin.
OriGene
Catalog No. ABIN3380979
Supplier Product No.: sc122190
$297.00
Plus shipping costs $50.00
10 μg
Shipping to: United States
Delivery in 4 to 9 Business Days

Quick Overview for Human CACNG7 cDNA Clone in Mammalian Expression Vector (ABIN3380979)

Gene

CACNG7 (Calcium Channel, Voltage-Dependent, gamma Subunit 7 (CACNG7))

Application

Protein Expression (PExp)

Insert

cDNA

Vector

Mammalian Expression Vector

Vector Backbone

pCMV6-XL4

Promoter

Enhanced CMV Promoter, T7 Promoter

Bacterial Resistance

Ampicillin

Expression Type

Transient
  • Species

    Human

    Supplier Product No.

    sc122190

    Supplier

    OriGene

    Purpose

    Untagged full-length cDNA clone from Human CACNG7 is ideal for over-expression of native protein for functional studies.

    Characteristics

    • These cDNA clones are isolated from full-length cDNA libraries and usually contain the coding sequence as well as the untranslated regions (UTRs) of the mRNA transcript appropriate to the library from which they were isolated.
    • These cDNA clones are ideal for over-expression of native proteins for functional studies. Provided as 10 μg transfection-ready plasmids.
    • Every lot of primer is tested to provide clean sequencing of cDNA clones.

    Purification

    The DNAs were purified using PowerPrep HP Plasmid isolation kits for transfection ready plasmids.

    Components

    • The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA.
    • The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

    Insert Length

    1200 bp

    Sequencing Primer

    VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3', XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
  • Restrictions

    For Research Use only
  • Format

    Lyophilized

    Storage

    RT,-20 °C

    Storage Comment

    The lyophilized plasmid is stable for up to one year when stored at ambient temperature. Following dissolution in 100 μL dH2O, store at -20 °C. Lyophilized primers are stable for up to one year when stored at ambient temperature. Following dissolution in 10 μL dH2O, store at -20 °C.

    Expiry Date

    12 months
  • Studniarczyk, Coombs, Cull-Candy, Farrant: "TARP γ-7 selectively enhances synaptic expression of calcium-permeable AMPARs." in: Nature neuroscience, Vol. 16, Issue 9, pp. 1266-74, (2013) (PubMed).

    Soto, Coombs, Renzi, Zonouzi, Farrant, Cull-Candy: "Selective regulation of long-form calcium-permeable AMPA receptors by an atypical TARP, gamma-5." in: Nature neuroscience, Vol. 12, Issue 3, pp. 277-85, (2009) (PubMed).

  • Target

    CACNG7 (Calcium Channel, Voltage-Dependent, gamma Subunit 7 (CACNG7))

    Alternative Name

    CACNG7

    Background

    The protein encoded by this gene is a type II transmembrane AMPA receptor regulatory protein (TARP). TARPs regulate both trafficking and channel gating of the AMPA receptors. This gene is part of a functionally diverse eight-member protein subfamily of the PMP-22/EMP/MP20 family and is located in a cluster with two family members, a type I TARP and a calcium channel gamma subunit. [provided by RefSeq, Dec 2010].

    NCBI Accession

    NM_031896, NP_114102
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