Human GAB2 cDNA Clone in Mammalian Expression Vector
This is a GRB2-Associated Binding Protein 2 plasmid from OriGene - one-time cited - with cDNA insert cloned into Mammalian Expression VectorpCMV6-XL4. Insert length: 2700 bp. Transient expression. Suitable for PExp.
Bacterial selection: Ampicillin.
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(1 reference)
Catalog No. ABIN3381221
Supplier Product No.: sc305788
$613.80
Plus shipping costs $50.00
10 μg
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United States
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Quick Overview for Human GAB2 cDNA Clone in Mammalian Expression Vector (ABIN3381221)
Gene
GAB2
(GRB2-Associated Binding Protein 2 (GAB2))
Application
Protein Expression (PExp)
Insert
cDNA
Vector
Mammalian Expression Vector
Vector Backbone
pCMV6-XL4
Promoter
Enhanced CMV Promoter, T7 Promoter
Bacterial Resistance
Ampicillin
Expression Type
Transient
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Species
- Human
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Supplier Product No.
- sc305788
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Supplier
- OriGene
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Purpose
- Untagged full-length cDNA clone from Human GAB2 is ideal for over-expression of native protein for functional studies.
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Characteristics
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- These cDNA clones are isolated from full-length cDNA libraries and usually contain the coding sequence as well as the untranslated regions (UTRs) of the mRNA transcript appropriate to the library from which they were isolated.
- These cDNA clones are ideal for over-expression of native proteins for functional studies. Provided as 10 μg transfection-ready plasmids.
- Every lot of primer is tested to provide clean sequencing of cDNA clones.
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Purification
- The DNAs were purified using PowerPrep HP Plasmid isolation kits for transfection ready plasmids.
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Components
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- The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA.
- The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
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Insert Length
- 2700 bp
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Sequencing Primer
- VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3', XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Storage
- RT,-20 °C
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Storage Comment
- The lyophilized plasmid is stable for up to one year when stored at ambient temperature. Following dissolution in 100 μL dH2O, store at -20 °C. Lyophilized primers are stable for up to one year when stored at ambient temperature. Following dissolution in 10 μL dH2O, store at -20 °C.
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Expiry Date
- 12 months
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: "Gab2-mediated signaling promotes melanoma metastasis." in: The American journal of pathology, Vol. 174, Issue 4, pp. 1524-33, (2009) (PubMed).
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: "Gab2-mediated signaling promotes melanoma metastasis." in: The American journal of pathology, Vol. 174, Issue 4, pp. 1524-33, (2009) (PubMed).
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- GAB2 (GRB2-Associated Binding Protein 2 (GAB2))
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Alternative Name
- GAB2
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Background
- This gene is a member of the GRB2-associated binding protein (GAB) gene family. These proteins contain pleckstrin homology (PH) domain, and bind SHP2 tyrosine phosphatase and GRB2 adapter protein. They act as adapters for transmitting various signals in response to stimuli through cytokine and growth factor receptors, and T- and B-cell antigen receptors. The protein encoded by this gene is the principal activator of phosphatidylinositol-3 kinase in response to activation of the high affinity IgE receptor. Two alternatively spliced transcripts encoding different isoforms have been described for this gene. [provided by RefSeq, Nov 2009].Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a).
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NCBI Accession
- NM_080491, NP_536739
Target
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