Human GABRB2 cDNA Clone in Mammalian Expression Vector
Quick Overview for Human GABRB2 cDNA Clone in Mammalian Expression Vector (ABIN3381222)
Gene
Application
Insert
Vector
Vector Backbone
Promoter
Bacterial Resistance
Expression Type
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Species
- Human
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Supplier Product No.
- sc308814
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Supplier
- OriGene
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Purpose
- Untagged full-length cDNA clone from Human GABRB2 is ideal for over-expression of native protein for functional studies.
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Characteristics
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- These cDNA clones are isolated from full-length cDNA libraries and usually contain the coding sequence as well as the untranslated regions (UTRs) of the mRNA transcript appropriate to the library from which they were isolated.
- These cDNA clones are ideal for over-expression of native proteins for functional studies. Provided as 10 μg transfection-ready plasmids.
- Every lot of primer is tested to provide clean sequencing of cDNA clones.
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Purification
- The DNAs were purified using PowerPrep HP Plasmid isolation kits for transfection ready plasmids.
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Components
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- The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA.
- The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
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Sequencing Primer
- VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3', XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Storage
- RT,-20 °C
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Storage Comment
- The lyophilized plasmid is stable for up to one year when stored at ambient temperature. Following dissolution in 100 μL dH2O, store at -20 °C. Lyophilized primers are stable for up to one year when stored at ambient temperature. Following dissolution in 10 μL dH2O, store at -20 °C.
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Expiry Date
- 12 months
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: "Multiple novel modes of action involved in the in vitro neurotoxic effects of tetrabromobisphenol-A." in: Toxicological sciences : an official journal of the Society of Toxicology, Vol. 128, Issue 1, pp. 235-46, (2012) (PubMed).
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: "Multiple novel modes of action involved in the in vitro neurotoxic effects of tetrabromobisphenol-A." in: Toxicological sciences : an official journal of the Society of Toxicology, Vol. 128, Issue 1, pp. 235-46, (2012) (PubMed).
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- GABRB2 (gamma-aminobutyric Acid (GABA) A Receptor, beta 2 (GABRB2))
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Alternative Name
- GABRB2
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Background
- The gamma-aminobutyric acid (GABA) A receptor is a multisubunit chloride channel that mediates the fastest inhibitory synaptic transmission in the central nervous system. This gene encodes GABA A receptor, beta 2 subunit. It is mapped to chromosome 5q34 in a cluster comprised of genes encoding alpha 1 and gamma 2 subunits of the GABA A receptor. Alternative splicing of this gene generates 2 transcript variants, differing by a 114 bp insertion. [provided by RefSeq, Jul 2008].Transcript Variant: This variant (1) encodes the full length isoform.
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NCBI Accession
- NM_021911, NP_068711
Target
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