Human GATA4 cDNA Clone in Mammalian Expression Vector
This is a GATA Binding Protein 4 plasmid from OriGene - 4 times cited - with cDNA insert cloned into Mammalian Expression VectorpCMV6-XL4. Insert length: 3600 bp. Transient expression. Suitable for PExp.
Bacterial selection: Ampicillin.
1 image
(4 references)
Catalog No. ABIN3381231
Supplier Product No.: sc124037
$452.43
Plus shipping costs $50.00
10 μg
Shipping to:
United States
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Quick Overview for Human GATA4 cDNA Clone in Mammalian Expression Vector (ABIN3381231)
Gene
GATA4
(GATA Binding Protein 4 (GATA4))
Application
Protein Expression (PExp)
Insert
cDNA
Vector
Mammalian Expression Vector
Vector Backbone
pCMV6-XL4
Promoter
Enhanced CMV Promoter, T7 Promoter
Bacterial Resistance
Ampicillin
Expression Type
Transient
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Species
- Human
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Supplier Product No.
- sc124037
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Supplier
- OriGene
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Purpose
- Untagged full-length cDNA clone from Human GATA4 is ideal for over-expression of native protein for functional studies.
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Characteristics
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- These cDNA clones are isolated from full-length cDNA libraries and usually contain the coding sequence as well as the untranslated regions (UTRs) of the mRNA transcript appropriate to the library from which they were isolated.
- These cDNA clones are ideal for over-expression of native proteins for functional studies. Provided as 10 μg transfection-ready plasmids.
- Every lot of primer is tested to provide clean sequencing of cDNA clones.
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Purification
- The DNAs were purified using PowerPrep HP Plasmid isolation kits for transfection ready plasmids.
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Components
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- The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA.
- The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
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Insert Length
- 3600 bp
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Sequencing Primer
- VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3', XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Storage
- RT,-20 °C
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Storage Comment
- The lyophilized plasmid is stable for up to one year when stored at ambient temperature. Following dissolution in 100 μL dH2O, store at -20 °C. Lyophilized primers are stable for up to one year when stored at ambient temperature. Following dissolution in 10 μL dH2O, store at -20 °C.
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Expiry Date
- 12 months
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: "Loss of GATA5 expression due to gene promoter methylation induces growth and colony formation of hepatocellular carcinoma cells." in: Oncology letters, Vol. 11, Issue 1, pp. 861-869, (2016) (PubMed).
: "Direct reprogramming of human fibroblasts to hepatocyte-like cells by synthetic modified mRNAs." in: PLoS ONE, Vol. 9, Issue 6, pp. e100134, (2014) (PubMed).
: "A GATA4-regulated tumor suppressor network represses formation of malignant human astrocytomas." in: The Journal of experimental medicine, Vol. 208, Issue 4, pp. 689-702, (2011) (PubMed).
: "GATA4 is a regulator of astrocyte cell proliferation and apoptosis in the human and murine central nervous system." in: Oncogene, Vol. 28, Issue 34, pp. 3033-46, (2009) (PubMed).
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- GATA4 (GATA Binding Protein 4 (GATA4))
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Alternative Name
- GATA4
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Background
- This gene encodes a member of the GATA family of zinc-finger transcription factors. Members of this family recognize the GATA motif which is present in the promoters of many genes. This protein is thought to regulate genes involved in embryogenesis and in myocardial differentiation and function, and is necessary for normal testicular development. Mutations in this gene have been associated with cardiac septal defects. Additionally, alterations in gene expression have been associated with several cancer types. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2015].
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NCBI Accession
- NM_002052, NP_002043
Target
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