Human HDAC4 cDNA Clone in Mammalian Expression Vector
This is a Histone Deacetylase 4 plasmid from OriGene - 2 times cited - with cDNA insert cloned into Mammalian Expression VectorpCMV6-XL4. Insert length: 4700 bp. Transient expression. Suitable for PExp.
Bacterial selection: Ampicillin.
1 image
(2 references)
Catalog No. ABIN3381308
Supplier Product No.: sc125507
$1,401.84
Plus shipping costs $50.00
10 μg
Shipping to:
United States
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Quick Overview for Human HDAC4 cDNA Clone in Mammalian Expression Vector (ABIN3381308)
Gene
HDAC4
(Histone Deacetylase 4 (HDAC4))
Application
Protein Expression (PExp)
Insert
cDNA
Vector
Mammalian Expression Vector
Vector Backbone
pCMV6-XL4
Promoter
Enhanced CMV Promoter, T7 Promoter
Bacterial Resistance
Ampicillin
Expression Type
Transient
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Species
- Human
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Supplier Product No.
- sc125507
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Supplier
- OriGene
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Purpose
- Untagged full-length cDNA clone from Human HDAC4 is ideal for over-expression of native protein for functional studies.
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Characteristics
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- These cDNA clones are isolated from full-length cDNA libraries and usually contain the coding sequence as well as the untranslated regions (UTRs) of the mRNA transcript appropriate to the library from which they were isolated.
- These cDNA clones are ideal for over-expression of native proteins for functional studies. Provided as 10 μg transfection-ready plasmids.
- Every lot of primer is tested to provide clean sequencing of cDNA clones.
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Purification
- The DNAs were purified using PowerPrep HP Plasmid isolation kits for transfection ready plasmids.
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Components
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- The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA.
- The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
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Insert Length
- 4700 bp
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Sequencing Primer
- VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3', XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Storage
- RT,-20 °C
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Storage Comment
- The lyophilized plasmid is stable for up to one year when stored at ambient temperature. Following dissolution in 100 μL dH2O, store at -20 °C. Lyophilized primers are stable for up to one year when stored at ambient temperature. Following dissolution in 10 μL dH2O, store at -20 °C.
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Expiry Date
- 12 months
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: "Proteomics analysis of human obesity reveals the epigenetic factor HDAC4 as a potential target for obesity." in: PLoS ONE, Vol. 8, Issue 9, pp. e75342, (2013) (PubMed).
: "HDAC4 reduction: a novel therapeutic strategy to target cytoplasmic huntingtin and ameliorate neurodegeneration." in: PLoS biology, Vol. 11, Issue 11, pp. e1001717, (2013) (PubMed).
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: "Proteomics analysis of human obesity reveals the epigenetic factor HDAC4 as a potential target for obesity." in: PLoS ONE, Vol. 8, Issue 9, pp. e75342, (2013) (PubMed).
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- HDAC4 (Histone Deacetylase 4 (HDAC4))
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Alternative Name
- HDAC4
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Background
- Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to class II of the histone deacetylase/acuc/apha family. It possesses histone deacetylase activity and represses transcription when tethered to a promoter. This protein does not bind DNA directly, but through transcription factors MEF2C and MEF2D. It seems to interact in a multiprotein complex with RbAp48 and HDAC3. [provided by RefSeq, Jul 2008].
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NCBI Accession
- NM_006037, NP_006028
Target
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