Human HES5 cDNA Clone in Mammalian Expression Vector
This is a Hairy and Enhancer of Split 5 plasmid from OriGene - 3 times cited - with cDNA insert cloned into Mammalian Expression VectorpCMV6-XL4. Insert length: 1300 bp. Transient expression. Suitable for PExp.
Bacterial selection: Ampicillin.
1 image
(3 references)
Catalog No. ABIN3381311
Supplier Product No.: sc301536
$297.00
Plus shipping costs $50.00
10 μg
Shipping to:
United States
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Quick Overview for Human HES5 cDNA Clone in Mammalian Expression Vector (ABIN3381311)
Gene
HES5
(Hairy and Enhancer of Split 5 (HES5))
Application
Protein Expression (PExp)
Insert
cDNA
Vector
Mammalian Expression Vector
Vector Backbone
pCMV6-XL4
Promoter
Enhanced CMV Promoter, T7 Promoter
Bacterial Resistance
Ampicillin
Expression Type
Transient
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Species
- Human
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Supplier Product No.
- sc301536
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Supplier
- OriGene
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Purpose
- Untagged full-length cDNA clone from Human HES5 is ideal for over-expression of native protein for functional studies.
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Characteristics
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- These cDNA clones are isolated from full-length cDNA libraries and usually contain the coding sequence as well as the untranslated regions (UTRs) of the mRNA transcript appropriate to the library from which they were isolated.
- These cDNA clones are ideal for over-expression of native proteins for functional studies. Provided as 10 μg transfection-ready plasmids.
- Every lot of primer is tested to provide clean sequencing of cDNA clones.
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Purification
- The DNAs were purified using PowerPrep HP Plasmid isolation kits for transfection ready plasmids.
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Components
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- The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA.
- The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
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Insert Length
- 1300 bp
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Sequencing Primer
- VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3', XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Storage
- RT,-20 °C
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Storage Comment
- The lyophilized plasmid is stable for up to one year when stored at ambient temperature. Following dissolution in 100 μL dH2O, store at -20 °C. Lyophilized primers are stable for up to one year when stored at ambient temperature. Following dissolution in 10 μL dH2O, store at -20 °C.
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Expiry Date
- 12 months
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: "Transcription factors with conserved binding sites near ATOH1 on the POU4F3 gene enhance the induction of cochlear hair cells." in: Molecular neurobiology, Vol. 51, Issue 2, pp. 672-84, (2015) (PubMed).
: "HES5 is a key mediator of Wnt-3a-induced neuronal differentiation." in: Stem cells and development, Vol. 23, Issue 12, pp. 1328-39, (2014) (PubMed).
: "Notch-1 stimulates survival of lung adenocarcinoma cells during hypoxia by activating the IGF-1R pathway." in: Oncogene, Vol. 29, Issue 17, pp. 2488-98, (2010) (PubMed).
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- HES5 (Hairy and Enhancer of Split 5 (HES5))
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Alternative Name
- HES5
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Background
- This gene encodes a member of a family of basic helix-loop-helix transcriptional repressors. The protein product of this gene, which is activated downstream of the Notch pathway, regulates cell differentiation in multiple tissues. Disruptions in the normal expression of this gene have been associated with developmental diseases and cancer. [provided by RefSeq, Dec 2008].
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NCBI Accession
- NM_001010926, NP_001010926
Target
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