Human IGF1R cDNA Clone in Mammalian Expression Vector
This is a Insulin-Like Growth Factor 1 Receptor plasmid from OriGene - 2 times cited - with cDNA insert cloned into Mammalian Expression VectorpCMV6-XL4. Insert length: 5500 bp. Transient expression. Suitable for PExp.
Bacterial selection: Ampicillin.
1 image
(2 references)
Catalog No. ABIN3381350
Supplier Product No.: sc124152
$1,737.45
Plus shipping costs $50.00
10 μg
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United States
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Quick Overview for Human IGF1R cDNA Clone in Mammalian Expression Vector (ABIN3381350)
Gene
IGF1R
(Insulin-Like Growth Factor 1 Receptor (IGF1R))
Application
Protein Expression (PExp)
Insert
cDNA
Vector
Mammalian Expression Vector
Vector Backbone
pCMV6-XL4
Promoter
Enhanced CMV Promoter, T7 Promoter
Bacterial Resistance
Ampicillin
Expression Type
Transient
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Species
- Human
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Supplier Product No.
- sc124152
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Supplier
- OriGene
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Purpose
- Untagged full-length cDNA clone from Human IGF1R is ideal for over-expression of native protein for functional studies.
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Characteristics
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- These cDNA clones are isolated from full-length cDNA libraries and usually contain the coding sequence as well as the untranslated regions (UTRs) of the mRNA transcript appropriate to the library from which they were isolated.
- These cDNA clones are ideal for over-expression of native proteins for functional studies. Provided as 10 μg transfection-ready plasmids.
- Every lot of primer is tested to provide clean sequencing of cDNA clones.
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Purification
- The DNAs were purified using PowerPrep HP Plasmid isolation kits for transfection ready plasmids.
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Components
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- The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA.
- The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
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Insert Length
- 5500 bp
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Sequencing Primer
- VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3', XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Storage
- RT,-20 °C
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Storage Comment
- The lyophilized plasmid is stable for up to one year when stored at ambient temperature. Following dissolution in 100 μL dH2O, store at -20 °C. Lyophilized primers are stable for up to one year when stored at ambient temperature. Following dissolution in 10 μL dH2O, store at -20 °C.
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Expiry Date
- 12 months
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: "PTEN regulates IGF-1R-mediated therapy resistance in melanoma." in: Pigment cell & melanoma research, Vol. 28, Issue 5, pp. 572-89, (2015) (PubMed).
: "Insulin-like growth factor 1 receptor and response to anti-IGF1R antibody therapy in osteosarcoma." in: PLoS ONE, Vol. 9, Issue 8, pp. e106249, (2014) (PubMed).
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- IGF1R (Insulin-Like Growth Factor 1 Receptor (IGF1R))
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Alternative Name
- IGF1R
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Background
- This receptor binds insulin-like growth factor with a high affinity. It has tyrosine kinase activity. The insulin-like growth factor I receptor plays a critical role in transformation events. Cleavage of the precursor generates alpha and beta subunits. It is highly overexpressed in most malignant tissues where it functions as an anti-apoptotic agent by enhancing cell survival. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, May 2014].Transcript Variant: This variant (1) encodes the longer isoform (1).
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NCBI Accession
- NM_000875, NP_000866
Target
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