Human SCN3A cDNA Clone in Mammalian Expression Vector
This is a Sodium Channel, Voltage-Gated, Type III, alpha Subunit plasmid from OriGene - 2 times cited - with cDNA insert cloned into Mammalian Expression VectorpCMV6-XL4. Insert length: 8900 bp. Transient expression. Suitable for PExp.
Bacterial selection: Ampicillin.
1 image
(2 references)
Catalog No. ABIN3381758
Supplier Product No.: sc316038
$2,435.40
Plus shipping costs $50.00
10 μg
Shipping to:
United States
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Quick Overview for Human SCN3A cDNA Clone in Mammalian Expression Vector (ABIN3381758)
Gene
SCN3A
(Sodium Channel, Voltage-Gated, Type III, alpha Subunit (SCN3A))
Application
Protein Expression (PExp)
Insert
cDNA
Vector
Mammalian Expression Vector
Vector Backbone
pCMV6-XL4
Promoter
Enhanced CMV Promoter, T7 Promoter
Bacterial Resistance
Ampicillin
Expression Type
Transient
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Species
- Human
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Supplier Product No.
- sc316038
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Supplier
- OriGene
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Purpose
- Untagged full-length cDNA clone from Human SCN3A is ideal for over-expression of native protein for functional studies.
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Characteristics
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- These cDNA clones are isolated from full-length cDNA libraries and usually contain the coding sequence as well as the untranslated regions (UTRs) of the mRNA transcript appropriate to the library from which they were isolated.
- These cDNA clones are ideal for over-expression of native proteins for functional studies. Provided as 10 μg transfection-ready plasmids.
- Every lot of primer is tested to provide clean sequencing of cDNA clones.
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Purification
- The DNAs were purified using PowerPrep HP Plasmid isolation kits for transfection ready plasmids.
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Components
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- The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA.
- The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
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Insert Length
- 8900 bp
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Sequencing Primer
- VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3', XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Storage
- RT,-20 °C
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Storage Comment
- The lyophilized plasmid is stable for up to one year when stored at ambient temperature. Following dissolution in 100 μL dH2O, store at -20 °C. Lyophilized primers are stable for up to one year when stored at ambient temperature. Following dissolution in 10 μL dH2O, store at -20 °C.
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Expiry Date
- 12 months
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: "Electrophysiological Differences between the Same Pore Region Mutation in SCN1A and SCN3A." in: Molecular neurobiology, Vol. 51, Issue 3, pp. 1263-70, (2015) (PubMed).
: "The Scorpion Toxin Tf2 from Tityus fasciolatus Promotes Nav1.3 Opening." in: PLoS ONE, Vol. 10, Issue 6, pp. e0128578, (2015) (PubMed).
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- SCN3A (Sodium Channel, Voltage-Gated, Type III, alpha Subunit (SCN3A))
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Alternative Name
- SCN3A
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Background
- Voltage-gated sodium channels are transmembrane glycoprotein complexes composed of a large alpha subunit with 24 transmembrane domains and one or more regulatory beta subunits. They are responsible for the generation and propagation of action potentials in neurons and muscle. This gene encodes one member of the sodium channel alpha subunit gene family, and is found in a cluster of five alpha subunit genes on chromosome 2. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008].Transcript Variant: This variant (3), also known as the neonatal form, uses an alternate in-frame splice site in the central coding region and an alternate form of an exon in the 5' coding region, compared to variant 1. The resulting isoform (3) is shorter than isoform 1, and contains one amino acid substitution relative to isoform 2.
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NCBI Accession
- NM_001081677, NP_001075146
Target
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