Human Src cDNA Clone in Mammalian Expression Vector
This is a Proto-oncogene tyrosine-protein kinase Src plasmid from OriGene - 2 times cited - with cDNA insert cloned into Mammalian Expression VectorpCMV6-XL4. Insert length: 5500 bp. Transient expression. Suitable for PExp.
Bacterial selection: Ampicillin.
1 image
(2 references)
Catalog No. ABIN3381815
Supplier Product No.: sc125208
$495.00
Plus shipping costs $50.00
10 μg
Shipping to:
United States
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Quick Overview for Human Src cDNA Clone in Mammalian Expression Vector (ABIN3381815)
Gene
Src
(Proto-oncogene tyrosine-protein kinase Src (Src))
Application
Protein Expression (PExp)
Insert
cDNA
Vector
Mammalian Expression Vector
Vector Backbone
pCMV6-XL4
Promoter
Enhanced CMV Promoter, T7 Promoter
Bacterial Resistance
Ampicillin
Expression Type
Transient
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Species
- Human
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Supplier Product No.
- sc125208
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Supplier
- OriGene
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Purpose
- Untagged full-length cDNA clone from Human SRC is ideal for over-expression of native protein for functional studies.
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Characteristics
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- These cDNA clones are isolated from full-length cDNA libraries and usually contain the coding sequence as well as the untranslated regions (UTRs) of the mRNA transcript appropriate to the library from which they were isolated.
- These cDNA clones are ideal for over-expression of native proteins for functional studies. Provided as 10 μg transfection-ready plasmids.
- Every lot of primer is tested to provide clean sequencing of cDNA clones.
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Purification
- The DNAs were purified using PowerPrep HP Plasmid isolation kits for transfection ready plasmids.
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Components
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- The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA.
- The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
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Insert Length
- 5500 bp
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Sequencing Primer
- VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3', XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Storage
- RT,-20 °C
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Storage Comment
- The lyophilized plasmid is stable for up to one year when stored at ambient temperature. Following dissolution in 100 μL dH2O, store at -20 °C. Lyophilized primers are stable for up to one year when stored at ambient temperature. Following dissolution in 10 μL dH2O, store at -20 °C.
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Expiry Date
- 12 months
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: "Src tyrosine kinase phosphorylation of nuclear receptor HNF4α correlates with isoform-specific loss of HNF4α in human colon cancer." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 109, Issue 7, pp. 2302-7, (2012) (PubMed).
: "Frank-ter Haar syndrome protein Tks4 regulates epidermal growth factor-dependent cell migration." in: The Journal of biological chemistry, Vol. 287, Issue 37, pp. 31321-9, (2012) (PubMed).
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: "Src tyrosine kinase phosphorylation of nuclear receptor HNF4α correlates with isoform-specific loss of HNF4α in human colon cancer." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 109, Issue 7, pp. 2302-7, (2012) (PubMed).
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- Src (Proto-oncogene tyrosine-protein kinase Src (Src))
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Alternative Name
- SRC
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Target Type
- Viral Protein
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Background
- This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008].Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 both encode the same protein.
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NCBI Accession
- NM_005417, NP_005408
Target
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