Human ABCF1 cDNA Clone in Mammalian Expression Vector
This is a ATP-Binding Cassette, Sub-Family F (GCN20), Member 1 plasmid from OriGene with cDNA insert cloned into Mammalian Expression VectorpCMV6-XL5. Insert length: 3270 bp. Transient expression. Suitable for PExp.
Bacterial selection: Ampicillin.
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Catalog No. ABIN3382310
Supplier Product No.: sc119474
$732.60
Plus shipping costs $50.00
10 μg
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United States
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Quick Overview for Human ABCF1 cDNA Clone in Mammalian Expression Vector (ABIN3382310)
Gene
ABCF1
(ATP-Binding Cassette, Sub-Family F (GCN20), Member 1 (ABCF1))
Application
Protein Expression (PExp)
Insert
cDNA
Vector
Mammalian Expression Vector
Vector Backbone
pCMV6-XL5
Promoter
Enhanced CMV Promoter, T7 Promoter
Bacterial Resistance
Ampicillin
Expression Type
Transient
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Species
- Human
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Supplier Product No.
- sc119474
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Supplier
- OriGene
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Purpose
- Untagged full-length cDNA clone from Human ABCF1 is ideal for over-expression of native protein for functional studies.
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Specificity
- Restriction Site: NotI-NotI
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Characteristics
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- These cDNA clones are isolated from full-length cDNA libraries and usually contain the coding sequence as well as the untranslated regions (UTRs) of the mRNA transcript appropriate to the library from which they were isolated.
- These cDNA clones are ideal for over-expression of native proteins for functional studies. Provided as 10 μg transfection-ready plasmids.
- Every lot of primer is tested to provide clean sequencing of cDNA clones.
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Purification
- The DNAs were purified using PowerPrep HP Plasmid isolation kits for transfection ready plasmids.
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Components
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- The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA.
- The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
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Insert Length
- 3270 bp
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Sequencing Primer
- VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3', XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Storage
- RT,-20 °C
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Storage Comment
- The lyophilized plasmid is stable for up to one year when stored at ambient temperature. Following dissolution in 100 μL dH2O, store at -20 °C. Lyophilized primers are stable for up to one year when stored at ambient temperature. Following dissolution in 10 μL dH2O, store at -20 °C.
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Expiry Date
- 12 months
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- ABCF1 (ATP-Binding Cassette, Sub-Family F (GCN20), Member 1 (ABCF1))
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Alternative Name
- ABCF1
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Background
- The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the GCN20 subfamily. Unlike other members of the superfamily, this protein lacks the transmembrane domains which are characteristic of most ABC transporters. This protein may be regulated by tumor necrosis factor-alpha and play a role in enhancement of protein synthesis and the inflammation process. [provided by RefSeq, Jul 2008].Transcript Variant: This variant (2) lacks an alternate in-frame exon, compared to variant 1. The resulting protein (isoform b) is shorter than isoform a.
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NCBI Accession
- NM_001090, NP_001081
Target
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