Human ADRB2 cDNA Clone in Mammalian Expression Vector
This is a Adrenergic, beta-2-, Receptor, Surface plasmid from OriGene - 4 times cited - with cDNA insert cloned into Mammalian Expression VectorpCMV6-XL5. Insert length: 2050 bp. Transient expression. Suitable for PExp.
Bacterial selection: Ampicillin.
1 image
(4 references)
Catalog No. ABIN3382386
Supplier Product No.: sc116997
$679.14
Plus shipping costs $50.00
10 μg
Shipping to:
United States
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Quick Overview for Human ADRB2 cDNA Clone in Mammalian Expression Vector (ABIN3382386)
Gene
beta 2 Adrenergic Receptor (ADRB2)
(Adrenergic, beta-2-, Receptor, Surface (ADRB2))
Application
Protein Expression (PExp)
Insert
cDNA
Vector
Mammalian Expression Vector
Vector Backbone
pCMV6-XL5
Promoter
Enhanced CMV Promoter, T7 Promoter
Bacterial Resistance
Ampicillin
Expression Type
Transient
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Species
- Human
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Supplier Product No.
- sc116997
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Supplier
- OriGene
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Purpose
- Untagged full-length cDNA clone from Human ADRB2 is ideal for over-expression of native protein for functional studies.
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Specificity
- Restriction Site: NotI-NotI
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Characteristics
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- These cDNA clones are isolated from full-length cDNA libraries and usually contain the coding sequence as well as the untranslated regions (UTRs) of the mRNA transcript appropriate to the library from which they were isolated.
- These cDNA clones are ideal for over-expression of native proteins for functional studies. Provided as 10 μg transfection-ready plasmids.
- Every lot of primer is tested to provide clean sequencing of cDNA clones.
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Purification
- The DNAs were purified using PowerPrep HP Plasmid isolation kits for transfection ready plasmids.
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Components
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- The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA.
- The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
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Insert Length
- 2050 bp
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Sequencing Primer
- VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3', XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Storage
- RT,-20 °C
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Storage Comment
- The lyophilized plasmid is stable for up to one year when stored at ambient temperature. Following dissolution in 100 μL dH2O, store at -20 °C. Lyophilized primers are stable for up to one year when stored at ambient temperature. Following dissolution in 10 μL dH2O, store at -20 °C.
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Expiry Date
- 12 months
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: "Cell-free methods to produce structurally intact mammalian membrane proteins." in: Scientific reports, Vol. 6, pp. 30442, (2016) (PubMed).
: "Divergent label-free cell phenotypic pharmacology of ligands at the overexpressed β₂-adrenergic receptors." in: Scientific reports, Vol. 4, pp. 3828, (2014) (PubMed).
: "Adenosine A2A receptor is involved in cell surface expression of A2B receptor." in: The Journal of biological chemistry, Vol. 285, Issue 50, pp. 39271-88, (2010) (PubMed).
: "PKA phosphorylation of SUR2B subunit underscores vascular KATP channel activation by beta-adrenergic receptors." in: American journal of physiology. Regulatory, integrative and comparative physiology, Vol. 293, Issue 3, pp. R1205-14, (2007) (PubMed).
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: "Cell-free methods to produce structurally intact mammalian membrane proteins." in: Scientific reports, Vol. 6, pp. 30442, (2016) (PubMed).
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- beta 2 Adrenergic Receptor (ADRB2) (Adrenergic, beta-2-, Receptor, Surface (ADRB2))
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Alternative Name
- ADRB2
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Background
- This gene encodes beta-2-adrenergic receptor which is a member of the G protein-coupled receptor superfamily. This receptor is directly associated with one of its ultimate effectors, the class C L-type calcium channel Ca(V)1.2. This receptor-channel complex also contains a G protein, an adenylyl cyclase, cAMP-dependent kinase, and the counterbalancing phosphatase, PP2A. The assembly of the signaling complex provides a mechanism that ensures specific and rapid signaling by this G protein-coupled receptor. This gene is intronless. Different polymorphic forms, point mutations, and/or downregulation of this gene are associated with nocturnal asthma, obesity and type 2 diabetes. [provided by RefSeq, Jul 2008].
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NCBI Accession
- NM_000024, NP_000015
Target
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