Human AKAP7 cDNA Clone in Mammalian Expression Vector
This is a A Kinase (PRKA) Anchor Protein 7 plasmid from OriGene - one-time cited - with cDNA insert cloned into Mammalian Expression VectorpCMV6-XL5. Insert length: 2750 bp. Transient expression. Suitable for PExp.
Bacterial selection: Ampicillin.
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Catalog No. ABIN3382431
Supplier Product No.: sc110061
$148.50
Plus shipping costs $50.00
10 μg
Shipping to:
United States
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Quick Overview for Human AKAP7 cDNA Clone in Mammalian Expression Vector (ABIN3382431)
Gene
AKAP7
(A Kinase (PRKA) Anchor Protein 7 (AKAP7))
Application
Protein Expression (PExp)
Insert
cDNA
Vector
Mammalian Expression Vector
Vector Backbone
pCMV6-XL5
Promoter
Enhanced CMV Promoter, T7 Promoter
Bacterial Resistance
Ampicillin
Expression Type
Transient
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Species
- Human
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Supplier Product No.
- sc110061
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Supplier
- OriGene
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Purpose
- Untagged full-length cDNA clone from Human AKAP7 is ideal for over-expression of native protein for functional studies.
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Specificity
- Restriction Site: NotI-NotI
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Characteristics
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- These cDNA clones are isolated from full-length cDNA libraries and usually contain the coding sequence as well as the untranslated regions (UTRs) of the mRNA transcript appropriate to the library from which they were isolated.
- These cDNA clones are ideal for over-expression of native proteins for functional studies. Provided as 10 μg transfection-ready plasmids.
- Every lot of primer is tested to provide clean sequencing of cDNA clones.
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Purification
- The DNAs were purified using PowerPrep HP Plasmid isolation kits for transfection ready plasmids.
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Components
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- The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA.
- The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
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Insert Length
- 2750 bp
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Sequencing Primer
- VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3', XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Storage
- RT,-20 °C
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Storage Comment
- The lyophilized plasmid is stable for up to one year when stored at ambient temperature. Following dissolution in 100 μL dH2O, store at -20 °C. Lyophilized primers are stable for up to one year when stored at ambient temperature. Following dissolution in 10 μL dH2O, store at -20 °C.
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Expiry Date
- 12 months
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: "AKAP18:PKA-RIIα structure reveals crucial anchor points for recognition of regulatory subunits of PKA." in: The Biochemical journal, Vol. 473, Issue 13, pp. 1881-94, (2016) (PubMed).
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- AKAP7 (A Kinase (PRKA) Anchor Protein 7 (AKAP7))
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Alternative Name
- AKAP7
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Background
- This gene encodes a member of the A-kinase anchoring protein (AKAP) family, a group of functionally related proteins that bind to a regulatory subunit (RII) of cAMP-dependent protein kinase A (PKA) and target the enzyme to specific subcellular compartments. AKAPs have a common RII-binding domain, but contain different targeting motifs responsible for directing PKA to distinct intracellular locations. Three alternatively spliced transcript variants encoding different isoforms have been described.[provided by RefSeq, Apr 2011].Transcript Variant: This variant (beta) uses an alternate in-frame exon, compared to variant alpha. The encoded isoform (beta) is longer than isoform alpha.
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NCBI Accession
- NM_138633, NP_619539
Target
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