Human ARFIP1 cDNA Clone in Mammalian Expression Vector
Quick Overview for Human ARFIP1 cDNA Clone in Mammalian Expression Vector (ABIN3382567)
Gene
Application
Insert
Vector
Vector Backbone
Promoter
Bacterial Resistance
Expression Type
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Species
- Human
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Supplier Product No.
- sc108595
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Supplier
- OriGene
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Purpose
- Untagged full-length cDNA clone from Human ARFIP1 is ideal for over-expression of native protein for functional studies.
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Specificity
- Restriction Site: NotI-NotI
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Characteristics
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- These cDNA clones are isolated from full-length cDNA libraries and usually contain the coding sequence as well as the untranslated regions (UTRs) of the mRNA transcript appropriate to the library from which they were isolated.
- These cDNA clones are ideal for over-expression of native proteins for functional studies. Provided as 10 μg transfection-ready plasmids.
- Every lot of primer is tested to provide clean sequencing of cDNA clones.
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Purification
- The DNAs were purified using PowerPrep HP Plasmid isolation kits for transfection ready plasmids.
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Components
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- The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA.
- The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
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Insert Length
- 3030 bp
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Sequencing Primer
- VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3', XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Storage
- RT,-20 °C
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Storage Comment
- The lyophilized plasmid is stable for up to one year when stored at ambient temperature. Following dissolution in 100 μL dH2O, store at -20 °C. Lyophilized primers are stable for up to one year when stored at ambient temperature. Following dissolution in 10 μL dH2O, store at -20 °C.
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Expiry Date
- 12 months
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: "Small-molecule synergist of the Wnt/beta-catenin signaling pathway." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 104, Issue 18, pp. 7444-8, (2007) (PubMed).
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- ARFIP1 (ADP-Ribosylation Factor Interacting Protein 1 (ARFIP1))
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Alternative Name
- ARFIP1
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Background
- Putative target protein of ADP-ribosylation factor. [UniProtKB/Swiss-Prot Function]Transcript Variant: This variant (3) differs in the 5' UTR and lacks an in-frame exon in the central coding region, compared to variant 1. The encoded isoform (2) is shorter, compared to isoform 1. Variants 2, 3, and 6 encode the same isoform.
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NCBI Accession
- NM_014447, NP_055262
Target
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