Human ARPC5 cDNA Clone in Mammalian Expression Vector
This is a Actin Related Protein 2/3 Complex, Subunit 5, 16kDa plasmid from OriGene - one-time cited - with cDNA insert cloned into Mammalian Expression VectorpCMV6-XL5. Insert length: 1920 bp. Transient expression. Suitable for PExp.
Bacterial selection: Ampicillin.
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Catalog No. ABIN3382605
Supplier Product No.: sc112682
$148.50
Plus shipping costs $50.00
10 μg
Shipping to:
United States
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Quick Overview for Human ARPC5 cDNA Clone in Mammalian Expression Vector (ABIN3382605)
Gene
ARPC5
(Actin Related Protein 2/3 Complex, Subunit 5, 16kDa (ARPC5))
Application
Protein Expression (PExp)
Insert
cDNA
Vector
Mammalian Expression Vector
Vector Backbone
pCMV6-XL5
Promoter
Enhanced CMV Promoter, T7 Promoter
Bacterial Resistance
Ampicillin
Expression Type
Transient
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Species
- Human
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Supplier Product No.
- sc112682
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Supplier
- OriGene
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Purpose
- Untagged full-length cDNA clone from Human ARPC5 is ideal for over-expression of native protein for functional studies.
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Specificity
- Restriction Site: NotI-NotI
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Characteristics
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- These cDNA clones are isolated from full-length cDNA libraries and usually contain the coding sequence as well as the untranslated regions (UTRs) of the mRNA transcript appropriate to the library from which they were isolated.
- These cDNA clones are ideal for over-expression of native proteins for functional studies. Provided as 10 μg transfection-ready plasmids.
- Every lot of primer is tested to provide clean sequencing of cDNA clones.
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Purification
- The DNAs were purified using PowerPrep HP Plasmid isolation kits for transfection ready plasmids.
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Components
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- The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA.
- The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
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Insert Length
- 1920 bp
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Sequencing Primer
- VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3', XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Storage
- RT,-20 °C
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Storage Comment
- The lyophilized plasmid is stable for up to one year when stored at ambient temperature. Following dissolution in 100 μL dH2O, store at -20 °C. Lyophilized primers are stable for up to one year when stored at ambient temperature. Following dissolution in 10 μL dH2O, store at -20 °C.
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Expiry Date
- 12 months
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: "Interaction between microRNAs and actin-associated protein Arpc5 regulates translational suppression during male germ cell differentiation." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 109, Issue 15, pp. 5750-5, (2012) (PubMed).
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- ARPC5 (Actin Related Protein 2/3 Complex, Subunit 5, 16kDa (ARPC5))
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Alternative Name
- ARPC5
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Background
- This gene encodes one of seven subunits of the human Arp2/3 protein complex. The Arp2/3 protein complex has been implicated in the control of actin polymerization in cells and has been conserved through evolution. The exact role of the protein encoded by this gene, the p16 subunit, has yet to be determined. Alternatively spliced transcript variants encoding different isoforms have been observed for this gene. [provided by RefSeq, Jul 2012].Transcript Variant: This variant (1) represents the shorter transcript and encodes the shorter protein (isoform 1).
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NCBI Accession
- NM_005717, NP_005708
Target
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