Human ATF3 cDNA Clone in Mammalian Expression Vector
This is a Activating Transcription Factor 3 plasmid from OriGene - one-time cited - with cDNA insert cloned into Mammalian Expression VectorpCMV6-XL5. Insert length: 2000 bp. Transient expression. Suitable for PExp.
Bacterial selection: Ampicillin.
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(1 reference)
Catalog No. ABIN3382639
Supplier Product No.: sc108959
$297.00
Plus shipping costs $50.00
10 μg
Shipping to:
United States
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Quick Overview for Human ATF3 cDNA Clone in Mammalian Expression Vector (ABIN3382639)
Gene
ATF3
(Activating Transcription Factor 3 (ATF3))
Application
Protein Expression (PExp)
Insert
cDNA
Vector
Mammalian Expression Vector
Vector Backbone
pCMV6-XL5
Promoter
Enhanced CMV Promoter, T7 Promoter
Bacterial Resistance
Ampicillin
Expression Type
Transient
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Species
- Human
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Supplier Product No.
- sc108959
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Supplier
- OriGene
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Purpose
- Untagged full-length cDNA clone from Human ATF3 is ideal for over-expression of native protein for functional studies.
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Specificity
- Restriction Site: NotI-NotI
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Characteristics
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- These cDNA clones are isolated from full-length cDNA libraries and usually contain the coding sequence as well as the untranslated regions (UTRs) of the mRNA transcript appropriate to the library from which they were isolated.
- These cDNA clones are ideal for over-expression of native proteins for functional studies. Provided as 10 μg transfection-ready plasmids.
- Every lot of primer is tested to provide clean sequencing of cDNA clones.
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Purification
- The DNAs were purified using PowerPrep HP Plasmid isolation kits for transfection ready plasmids.
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Components
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- The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA.
- The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
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Insert Length
- 2000 bp
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Sequencing Primer
- VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3', XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Storage
- RT,-20 °C
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Storage Comment
- The lyophilized plasmid is stable for up to one year when stored at ambient temperature. Following dissolution in 100 μL dH2O, store at -20 °C. Lyophilized primers are stable for up to one year when stored at ambient temperature. Following dissolution in 10 μL dH2O, store at -20 °C.
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Expiry Date
- 12 months
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: "Knockdown of RyR3 enhances adiponectin expression through an atf3-dependent pathway." in: Endocrinology, Vol. 154, Issue 3, pp. 1117-29, (2013) (PubMed).
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- ATF3 (Activating Transcription Factor 3 (ATF3))
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Alternative Name
- ATF3
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Background
- This gene encodes a member of the mammalian activation transcription factor/cAMP responsive element-binding (CREB) protein family of transcription factors. This gene is induced by a variety of signals, including many of those encountered by cancer cells, and is involved in the complex process of cellular stress response. Multiple transcript variants encoding different isoforms have been found for this gene. It is possible that alternative splicing of this gene may be physiologically important in the regulation of target genes. [provided by RefSeq, Apr 2011].Transcript Variant: This variant (1) encodes the longest isoform (1), which represses rather than activates transcription from promoters with ATF-binding elements. Both variants 1 and 3 encode isoform 1.
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NCBI Accession
- NM_001674, NP_001665
Target
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