Human ATP1A1 cDNA Clone in Mammalian Expression Vector
This is a Sodium Potassium ATPase, alpha1 plasmid from OriGene - 3 times cited - with cDNA insert cloned into Mammalian Expression VectorpCMV6-XL5. Insert length: 4040 bp. Transient expression. Suitable for PExp.
Bacterial selection: Ampicillin.
1 image
(3 references)
Catalog No. ABIN3382650
Supplier Product No.: sc119714
$882.09
Plus shipping costs $50.00
10 μg
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United States
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Quick Overview for Human ATP1A1 cDNA Clone in Mammalian Expression Vector (ABIN3382650)
Gene
ATP1A1
(Sodium Potassium ATPase, alpha1 (ATP1A1))
Application
Protein Expression (PExp)
Insert
cDNA
Vector
Mammalian Expression Vector
Vector Backbone
pCMV6-XL5
Promoter
Enhanced CMV Promoter, T7 Promoter
Bacterial Resistance
Ampicillin
Expression Type
Transient
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Species
- Human
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Supplier Product No.
- sc119714
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Supplier
- OriGene
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Purpose
- Untagged full-length cDNA clone from Human ATP1A1 is ideal for over-expression of native protein for functional studies.
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Specificity
- Restriction Site: NotI-NotI
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Characteristics
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- These cDNA clones are isolated from full-length cDNA libraries and usually contain the coding sequence as well as the untranslated regions (UTRs) of the mRNA transcript appropriate to the library from which they were isolated.
- These cDNA clones are ideal for over-expression of native proteins for functional studies. Provided as 10 μg transfection-ready plasmids.
- Every lot of primer is tested to provide clean sequencing of cDNA clones.
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Purification
- The DNAs were purified using PowerPrep HP Plasmid isolation kits for transfection ready plasmids.
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Components
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- The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA.
- The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
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Insert Length
- 4040 bp
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Sequencing Primer
- VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3', XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Storage
- RT,-20 °C
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Storage Comment
- The lyophilized plasmid is stable for up to one year when stored at ambient temperature. Following dissolution in 100 μL dH2O, store at -20 °C. Lyophilized primers are stable for up to one year when stored at ambient temperature. Following dissolution in 10 μL dH2O, store at -20 °C.
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Expiry Date
- 12 months
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: "Somatic mutations in ATP1A1 and CACNA1D underlie a common subtype of adrenal hypertension. ..." in: Nature genetics, Vol. 45, Issue 9, pp. 1055-60, (2013) (PubMed).
: "Reciprocal modulation of function between the D1 and D2 dopamine receptors and the Na+,K+-ATPase." in: The Journal of biological chemistry, Vol. 283, Issue 52, pp. 36441-53, (2008) (PubMed).
: "The structure of the Na+,K+-ATPase and mapping of isoform differences and disease-related mutations." in: Philosophical transactions of the Royal Society of London. Series B, Biological sciences, Vol. 364, Issue 1514, pp. 217-27, (2008) (PubMed).
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: "Somatic mutations in ATP1A1 and CACNA1D underlie a common subtype of adrenal hypertension. ..." in: Nature genetics, Vol. 45, Issue 9, pp. 1055-60, (2013) (PubMed).
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- ATP1A1 (Sodium Potassium ATPase, alpha1 (ATP1A1))
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Alternative Name
- ATP1A1
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Background
- The protein encoded by this gene belongs to the family of P-type cation transport ATPases, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The catalytic subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes an alpha 1 subunit. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009].Transcript Variant: This variant (1) represents use of an alternate promoter and 5' exon, compared to variant 3. The resulting isoform (a) is the same length but has a distinct N-terminus, compared to isoform c.
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NCBI Accession
- NM_000701, NP_000692
Target
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