Human AXIN1 cDNA Clone in Mammalian Expression Vector
This is a Axin 1 plasmid from OriGene - one-time cited - with cDNA insert cloned into Mammalian Expression VectorpCMV6-XL5. Insert length: 3500 bp. Transient expression. Suitable for PExp.
Bacterial selection: Ampicillin.
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(1 reference)
Catalog No. ABIN3382692
Supplier Product No.: sc303321
$782.10
Plus shipping costs $50.00
10 μg
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Quick Overview for Human AXIN1 cDNA Clone in Mammalian Expression Vector (ABIN3382692)
Gene
Axin (AXIN1)
(Axin 1 (AXIN1))
Application
Protein Expression (PExp)
Insert
cDNA
Vector
Mammalian Expression Vector
Vector Backbone
pCMV6-XL5
Promoter
Enhanced CMV Promoter, T7 Promoter
Bacterial Resistance
Ampicillin
Expression Type
Transient
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Species
- Human
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Supplier Product No.
- sc303321
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Supplier
- OriGene
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Purpose
- Untagged full-length cDNA clone from Human AXIN1 is ideal for over-expression of native protein for functional studies.
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Specificity
- Restriction Site: NotI-NotI
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Characteristics
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- These cDNA clones are isolated from full-length cDNA libraries and usually contain the coding sequence as well as the untranslated regions (UTRs) of the mRNA transcript appropriate to the library from which they were isolated.
- These cDNA clones are ideal for over-expression of native proteins for functional studies. Provided as 10 μg transfection-ready plasmids.
- Every lot of primer is tested to provide clean sequencing of cDNA clones.
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Purification
- The DNAs were purified using PowerPrep HP Plasmid isolation kits for transfection ready plasmids.
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Components
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- The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA.
- The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
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Insert Length
- 3500 bp
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Sequencing Primer
- VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3', XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Storage
- RT,-20 °C
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Storage Comment
- The lyophilized plasmid is stable for up to one year when stored at ambient temperature. Following dissolution in 100 μL dH2O, store at -20 °C. Lyophilized primers are stable for up to one year when stored at ambient temperature. Following dissolution in 10 μL dH2O, store at -20 °C.
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Expiry Date
- 12 months
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: "Human Sarcoma growth is sensitive to small-molecule mediated AXIN stabilization." in: PLoS ONE, Vol. 9, Issue 5, pp. e97847, (2014) (PubMed).
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: "Human Sarcoma growth is sensitive to small-molecule mediated AXIN stabilization." in: PLoS ONE, Vol. 9, Issue 5, pp. e97847, (2014) (PubMed).
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- Axin (AXIN1) (Axin 1 (AXIN1))
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Alternative Name
- AXIN1
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Background
- This gene encodes a cytoplasmic protein which contains a regulation of G-protein signaling (RGS) domain and a dishevelled and axin (DIX) domain. The encoded protein interacts with adenomatosis polyposis coli, catenin beta-1, glycogen synthase kinase 3 beta, protein phosphate 2, and itself. This protein functions as a negative regulator of the wingless-type MMTV integration site family, member 1 (WNT) signaling pathway and can induce apoptosis. The crystal structure of a portion of this protein, alone and in a complex with other proteins, has been resolved. Mutations in this gene have been associated with hepatocellular carcinoma, hepatoblastomas, ovarian endometriod adenocarcinomas, and medullablastomas. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2016].Transcript Variant: This variant (1) represents the longer transcript, and encodes the longer isoform (a). An upstream in-frame AUG is present that could extend the N-terminus of this isoform by 49 aa, however, there is no evidence that the upstream AUG is used, so the shorter N-terminus is being annotated.
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NCBI Accession
- NM_003502, NP_003493
Target
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