Human C1S cDNA Clone in Mammalian Expression Vector
This is a Complement Component 1, S Subcomponent plasmid from OriGene - one-time cited - with cDNA insert cloned into Mammalian Expression VectorpCMV6-XL5. Insert length: 2900 bp. Transient expression. Suitable for PExp.
Bacterial selection: Ampicillin.
1 image
(1 reference)
Catalog No. ABIN3382911
Supplier Product No.: sc126504
$624.69
Plus shipping costs $50.00
10 μg
Shipping to:
United States
Delivery in 4 to 9 Business Days
Quick Overview for Human C1S cDNA Clone in Mammalian Expression Vector (ABIN3382911)
Gene
C1S
(Complement Component 1, S Subcomponent (C1S))
Application
Protein Expression (PExp)
Insert
cDNA
Vector
Mammalian Expression Vector
Vector Backbone
pCMV6-XL5
Promoter
Enhanced CMV Promoter, T7 Promoter
Bacterial Resistance
Ampicillin
Expression Type
Transient
-
-
Species
- Human
-
Supplier Product No.
- sc126504
-
Supplier
- OriGene
-
Purpose
- Untagged full-length cDNA clone from Human C1S is ideal for over-expression of native protein for functional studies.
-
Specificity
- Restriction Site: NotI-NotI
-
Characteristics
-
- These cDNA clones are isolated from full-length cDNA libraries and usually contain the coding sequence as well as the untranslated regions (UTRs) of the mRNA transcript appropriate to the library from which they were isolated.
- These cDNA clones are ideal for over-expression of native proteins for functional studies. Provided as 10 μg transfection-ready plasmids.
- Every lot of primer is tested to provide clean sequencing of cDNA clones.
-
Purification
- The DNAs were purified using PowerPrep HP Plasmid isolation kits for transfection ready plasmids.
-
Components
-
- The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA.
- The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
-
Insert Length
- 2900 bp
-
Sequencing Primer
- VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3', XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
-
-
-
-
Restrictions
- For Research Use only
-
-
-
Format
- Lyophilized
-
Storage
- RT,-20 °C
-
Storage Comment
- The lyophilized plasmid is stable for up to one year when stored at ambient temperature. Following dissolution in 100 μL dH2O, store at -20 °C. Lyophilized primers are stable for up to one year when stored at ambient temperature. Following dissolution in 10 μL dH2O, store at -20 °C.
-
Expiry Date
- 12 months
-
-
-
: "Classical complement pathway components C1r and C1s: purification from human serum and in recombinant form and functional characterization." in: Methods in molecular biology (Clifton, N.J.), Vol. 1100, pp. 43-60, (2013) (PubMed).
-
: "Classical complement pathway components C1r and C1s: purification from human serum and in recombinant form and functional characterization." in: Methods in molecular biology (Clifton, N.J.), Vol. 1100, pp. 43-60, (2013) (PubMed).
-
- C1S (Complement Component 1, S Subcomponent (C1S))
-
Alternative Name
- C1S
-
Background
- This gene encodes a serine protease, which is a major constituent of the human complement subcomponent C1. C1s associates with two other complement components C1r and C1q in order to yield the first component of the serum complement system. Defects in this gene are the cause of selective C1s deficiency. [provided by RefSeq, Mar 2009].Transcript Variant: This variant (1) represents the longer transcript. Both variants 1 and 2 encode the same protein.
-
NCBI Accession
- NM_201442, NP_958850
Target
-
You are here:
