Human CELF1 cDNA Clone in Mammalian Expression Vector
This is a CUGBP, Elav-Like Family Member 1 plasmid from OriGene - 3 times cited - with cDNA insert cloned into Mammalian Expression VectorpCMV6-XL5. Insert length: 3780 bp. Transient expression. Suitable for PExp.
Bacterial selection: Ampicillin.
1 image
(3 references)
Catalog No. ABIN3383232
Supplier Product No.: sc128043
$452.43
Plus shipping costs $50.00
10 μg
Shipping to:
United States
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Quick Overview for Human CELF1 cDNA Clone in Mammalian Expression Vector (ABIN3383232)
Gene
CELF1
(CUGBP, Elav-Like Family Member 1 (CELF1))
Application
Protein Expression (PExp)
Insert
cDNA
Vector
Mammalian Expression Vector
Vector Backbone
pCMV6-XL5
Promoter
Enhanced CMV Promoter, T7 Promoter
Bacterial Resistance
Ampicillin
Expression Type
Transient
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Species
- Human
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Supplier Product No.
- sc128043
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Supplier
- OriGene
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Purpose
- Untagged full-length cDNA clone from Human CELF1 is ideal for over-expression of native protein for functional studies.
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Specificity
- Restriction Site: NotI-NotI
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Characteristics
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- These cDNA clones are isolated from full-length cDNA libraries and usually contain the coding sequence as well as the untranslated regions (UTRs) of the mRNA transcript appropriate to the library from which they were isolated.
- These cDNA clones are ideal for over-expression of native proteins for functional studies. Provided as 10 μg transfection-ready plasmids.
- Every lot of primer is tested to provide clean sequencing of cDNA clones.
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Purification
- The DNAs were purified using PowerPrep HP Plasmid isolation kits for transfection ready plasmids.
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Components
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- The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA.
- The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
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Insert Length
- 3780 bp
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Sequencing Primer
- VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3', XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Storage
- RT,-20 °C
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Storage Comment
- The lyophilized plasmid is stable for up to one year when stored at ambient temperature. Following dissolution in 100 μL dH2O, store at -20 °C. Lyophilized primers are stable for up to one year when stored at ambient temperature. Following dissolution in 10 μL dH2O, store at -20 °C.
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Expiry Date
- 12 months
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: "Competition between RNA-binding proteins CELF1 and HuR modulates MYC translation and intestinal epithelium renewal." in: Molecular biology of the cell, Vol. 26, Issue 10, pp. 1797-810, (2015) (PubMed).
: "Jnk2 deletion disrupts intestinal mucosal homeostasis and maturation by differentially modulating RNA-binding proteins HuR and CUGBP1." in: American journal of physiology. Cell physiology, Vol. 306, Issue 12, pp. C1167-75, (2014) (PubMed).
: "Competitive binding of CUGBP1 and HuR to occludin mRNA controls its translation and modulates epithelial barrier function." in: Molecular biology of the cell, Vol. 24, Issue 2, pp. 85-99, (2013) (PubMed).
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- CELF1 (CUGBP, Elav-Like Family Member 1 (CELF1))
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Alternative Name
- CELF1
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Background
- Members of the CELF/BRUNOL protein family contain two N-terminal RNA recognition motif (RRM) domains, one C-terminal RRM domain, and a divergent segment of 160-230 aa between the second and third RRM domains. Members of this protein family regulate pre-mRNA alternative splicing and may also be involved in mRNA editing, and translation. This gene may play a role in myotonic dystrophy type 1 (DM1) via interactions with the dystrophia myotonica-protein kinase (DMPK) gene. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008].Transcript Variant: This variant (1) is the predominant transcript and it encodes isoform 1.
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NCBI Accession
- NM_006560, NP_006551
Target
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