Human CFL1 cDNA Clone in Mammalian Expression Vector
This is a Cofilin 1 plasmid from OriGene - 3 times cited - with cDNA insert cloned into Mammalian Expression VectorpCMV6-XL5. Insert length: 1200 bp. Transient expression. Suitable for PExp.
Bacterial selection: Ampicillin.
1 image
(3 references)
Catalog No. ABIN3383254
Supplier Product No.: sc116694
$297.00
Plus shipping costs $50.00
10 μg
Shipping to:
United States
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Quick Overview for Human CFL1 cDNA Clone in Mammalian Expression Vector (ABIN3383254)
Gene
Cofilin (CFL1)
(Cofilin 1 (CFL1))
Application
Protein Expression (PExp)
Insert
cDNA
Vector
Mammalian Expression Vector
Vector Backbone
pCMV6-XL5
Promoter
Enhanced CMV Promoter, T7 Promoter
Bacterial Resistance
Ampicillin
Expression Type
Transient
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Species
- Human
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Supplier Product No.
- sc116694
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Supplier
- OriGene
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Purpose
- Untagged full-length cDNA clone from Human CFL1 is ideal for over-expression of native protein for functional studies.
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Specificity
- Restriction Site: NotI-NotI
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Characteristics
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- These cDNA clones are isolated from full-length cDNA libraries and usually contain the coding sequence as well as the untranslated regions (UTRs) of the mRNA transcript appropriate to the library from which they were isolated.
- These cDNA clones are ideal for over-expression of native proteins for functional studies. Provided as 10 μg transfection-ready plasmids.
- Every lot of primer is tested to provide clean sequencing of cDNA clones.
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Purification
- The DNAs were purified using PowerPrep HP Plasmid isolation kits for transfection ready plasmids.
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Components
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- The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA.
- The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
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Insert Length
- 1200 bp
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Sequencing Primer
- VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3', XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Storage
- RT,-20 °C
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Storage Comment
- The lyophilized plasmid is stable for up to one year when stored at ambient temperature. Following dissolution in 100 μL dH2O, store at -20 °C. Lyophilized primers are stable for up to one year when stored at ambient temperature. Following dissolution in 10 μL dH2O, store at -20 °C.
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Expiry Date
- 12 months
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: "The interactions between SATB1 and F-actin are important for mechanisms of active cell death." in: Folia histochemica et cytobiologica, Vol. 53, Issue 2, pp. 152-61, (2015) (PubMed).
: "Taurine chloramine-induced inactivation of cofilin protein through methionine oxidation." in: Free radical biology & medicine, Vol. 75, pp. 84-94, (2014) (PubMed).
: "Oxidant-induced apoptosis is mediated by oxidation of the actin-regulatory protein cofilin." in: Nature cell biology, Vol. 11, Issue 10, pp. 1241-6, (2009) (PubMed).
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- Cofilin (CFL1) (Cofilin 1 (CFL1))
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Alternative Name
- CFL1
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Background
- The protein encoded by this gene can polymerize and depolymerize F-actin and G-actin in a pH -dependent manner. Increased phosphorylation of this protein by LIM kinase aids in Rho-induced reorganization of the actin cytoskeleton. Cofilin is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH -dependent manner. It is involved in the translocation of actin-cofilin complex from cytoplasm to nucleus.[supplied by OMIM, Apr 2004].
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NCBI Accession
- NM_005507, NP_005498
Target
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