Human CXCR4 cDNA Clone in Mammalian Expression Vector
This is a Chemokine (C-X-C Motif) Receptor 4 plasmid from OriGene - one-time cited - with cDNA insert cloned into Mammalian Expression VectorpCMV6-XL5. Insert length: 1720 bp. Transient expression. Suitable for PExp.
Bacterial selection: Ampicillin.
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Catalog No. ABIN3383538
Supplier Product No.: sc117951
$679.14
Plus shipping costs $50.00
10 μg
Shipping to:
United States
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Quick Overview for Human CXCR4 cDNA Clone in Mammalian Expression Vector (ABIN3383538)
Gene
CXCR4
(Chemokine (C-X-C Motif) Receptor 4 (CXCR4))
Application
Protein Expression (PExp)
Insert
cDNA
Vector
Mammalian Expression Vector
Vector Backbone
pCMV6-XL5
Promoter
Enhanced CMV Promoter, T7 Promoter
Bacterial Resistance
Ampicillin
Expression Type
Transient
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Species
- Human
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Supplier Product No.
- sc117951
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Supplier
- OriGene
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Purpose
- Untagged full-length cDNA clone from Human CXCR4 is ideal for over-expression of native protein for functional studies.
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Specificity
- Restriction Site: NotI-NotI
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Characteristics
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- These cDNA clones are isolated from full-length cDNA libraries and usually contain the coding sequence as well as the untranslated regions (UTRs) of the mRNA transcript appropriate to the library from which they were isolated.
- These cDNA clones are ideal for over-expression of native proteins for functional studies. Provided as 10 μg transfection-ready plasmids.
- Every lot of primer is tested to provide clean sequencing of cDNA clones.
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Purification
- The DNAs were purified using PowerPrep HP Plasmid isolation kits for transfection ready plasmids.
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Components
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- The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA.
- The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
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Insert Length
- 1720 bp
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Sequencing Primer
- VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3', XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Storage
- RT,-20 °C
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Storage Comment
- The lyophilized plasmid is stable for up to one year when stored at ambient temperature. Following dissolution in 100 μL dH2O, store at -20 °C. Lyophilized primers are stable for up to one year when stored at ambient temperature. Following dissolution in 10 μL dH2O, store at -20 °C.
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Expiry Date
- 12 months
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: "CXCR4 Antagonists: A Screening Strategy for Identification of Functionally Selective Ligands." in: Journal of biomolecular screening, Vol. 19, Issue 6, pp. 859-69, (2016) (PubMed).
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: "CXCR4 Antagonists: A Screening Strategy for Identification of Functionally Selective Ligands." in: Journal of biomolecular screening, Vol. 19, Issue 6, pp. 859-69, (2016) (PubMed).
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- CXCR4 (Chemokine (C-X-C Motif) Receptor 4 (CXCR4))
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Alternative Name
- CXCR4
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Background
- This gene encodes a CXC chemokine receptor specific for stromal cell-derived factor-1. The protein has 7 transmembrane regions and is located on the cell surface. It acts with the CD4 protein to support HIV entry into cells and is also highly expressed in breast cancer cells. Mutations in this gene have been associated with WHIM (warts, hypogammaglobulinemia, infections, and myelokathexis) syndrome. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008].Transcript Variant: This variant (2) contains a distinct 5' UTR and lacks an in-frame portion of the 5' coding region, compared to variant 1. The resulting isoform (b) has a shorter N-terminus when compared to isoform a.
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NCBI Accession
- NM_003467, NP_003458
Target
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