Human EDNRA cDNA Clone in Mammalian Expression Vector
This is a Endothelin Receptor Type A plasmid from OriGene - 4 times cited - with cDNA insert cloned into Mammalian Expression VectorpCMV6-XL5. Insert length: 4290 bp. Transient expression. Suitable for PExp.
Bacterial selection: Ampicillin.
1 image
(4 references)
Catalog No. ABIN3383779
Supplier Product No.: sc118901
$452.43
Plus shipping costs $50.00
10 μg
Shipping to:
United States
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Quick Overview for Human EDNRA cDNA Clone in Mammalian Expression Vector (ABIN3383779)
Gene
Endothelin-1 Receptor (EDNRA)
(Endothelin Receptor Type A (EDNRA))
Application
Protein Expression (PExp)
Insert
cDNA
Vector
Mammalian Expression Vector
Vector Backbone
pCMV6-XL5
Promoter
Enhanced CMV Promoter, T7 Promoter
Bacterial Resistance
Ampicillin
Expression Type
Transient
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Species
- Human
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Supplier Product No.
- sc118901
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Supplier
- OriGene
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Purpose
- Untagged full-length cDNA clone from Human EDNRA is ideal for over-expression of native protein for functional studies.
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Specificity
- Restriction Site: NotI-NotI
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Characteristics
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- These cDNA clones are isolated from full-length cDNA libraries and usually contain the coding sequence as well as the untranslated regions (UTRs) of the mRNA transcript appropriate to the library from which they were isolated.
- These cDNA clones are ideal for over-expression of native proteins for functional studies. Provided as 10 μg transfection-ready plasmids.
- Every lot of primer is tested to provide clean sequencing of cDNA clones.
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Purification
- The DNAs were purified using PowerPrep HP Plasmid isolation kits for transfection ready plasmids.
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Components
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- The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA.
- The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
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Insert Length
- 4290 bp
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Sequencing Primer
- VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3', XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Storage
- RT,-20 °C
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Storage Comment
- The lyophilized plasmid is stable for up to one year when stored at ambient temperature. Following dissolution in 100 μL dH2O, store at -20 °C. Lyophilized primers are stable for up to one year when stored at ambient temperature. Following dissolution in 10 μL dH2O, store at -20 °C.
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Expiry Date
- 12 months
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: "Cell-free methods to produce structurally intact mammalian membrane proteins." in: Scientific reports, Vol. 6, pp. 30442, (2016) (PubMed).
: "Mutations in the endothelin receptor type A cause mandibulofacial dysostosis with alopecia. ..." in: American journal of human genetics, Vol. 96, Issue 4, pp. 519-31, (2015) (PubMed).
: "Desensitization and internalization of endothelin receptor A: impact of G protein-coupled receptor kinase 2 (GRK2)-mediated phosphorylation." in: The Journal of biological chemistry, Vol. 288, Issue 45, pp. 32138-48, (2013) (PubMed).
: "An antibody-drug conjugate targeting the endothelin B receptor for the treatment of melanoma." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 17, Issue 5, pp. 965-75, (2011) (PubMed).
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- Endothelin-1 Receptor (EDNRA) (Endothelin Receptor Type A (EDNRA))
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Alternative Name
- EDNRA
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Background
- This gene encodes the receptor for endothelin-1, a peptide that plays a role in potent and long-lasting vasoconstriction. This receptor associates with guanine-nucleotide-binding (G) proteins, and this coupling activates a phosphatidylinositol-calcium second messenger system. Polymorphisms in this gene have been linked to migraine headache resistance. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2009].Transcript Variant: This variant (1, also known as ET-AR) represents the longest transcript and encodes the longer isoform (a).
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NCBI Accession
- NM_001957, NP_001948
Target
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