Human ENPP2 cDNA Clone in Mammalian Expression Vector
This is a Ectonucleotide Pyrophosphatase / phosphodiesterase 2 plasmid from OriGene - one-time cited - with cDNA insert cloned into Mammalian Expression VectorpCMV6-XL5. Insert length: 3570 bp. Transient expression. Suitable for PExp.
Bacterial selection: Ampicillin.
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Catalog No. ABIN3383873
Supplier Product No.: sc116258
$830.61
Plus shipping costs $50.00
10 μg
Shipping to:
United States
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Quick Overview for Human ENPP2 cDNA Clone in Mammalian Expression Vector (ABIN3383873)
Gene
ENPP2
(Ectonucleotide Pyrophosphatase / phosphodiesterase 2 (ENPP2))
Application
Protein Expression (PExp)
Insert
cDNA
Vector
Mammalian Expression Vector
Vector Backbone
pCMV6-XL5
Promoter
Enhanced CMV Promoter, T7 Promoter
Bacterial Resistance
Ampicillin
Expression Type
Transient
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Species
- Human
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Supplier Product No.
- sc116258
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Supplier
- OriGene
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Purpose
- Untagged full-length cDNA clone from Human ENPP2 is ideal for over-expression of native protein for functional studies.
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Specificity
- Restriction Site: NotI-NotI
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Characteristics
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- These cDNA clones are isolated from full-length cDNA libraries and usually contain the coding sequence as well as the untranslated regions (UTRs) of the mRNA transcript appropriate to the library from which they were isolated.
- These cDNA clones are ideal for over-expression of native proteins for functional studies. Provided as 10 μg transfection-ready plasmids.
- Every lot of primer is tested to provide clean sequencing of cDNA clones.
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Purification
- The DNAs were purified using PowerPrep HP Plasmid isolation kits for transfection ready plasmids.
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Components
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- The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA.
- The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
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Insert Length
- 3570 bp
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Sequencing Primer
- VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3', XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Storage
- RT,-20 °C
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Storage Comment
- The lyophilized plasmid is stable for up to one year when stored at ambient temperature. Following dissolution in 100 μL dH2O, store at -20 °C. Lyophilized primers are stable for up to one year when stored at ambient temperature. Following dissolution in 10 μL dH2O, store at -20 °C.
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Expiry Date
- 12 months
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: "Polyoxometalates--potent and selective ecto-nucleotidase inhibitors." in: Biochemical pharmacology, Vol. 93, Issue 2, pp. 171-81, (2015) (PubMed).
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: "Polyoxometalates--potent and selective ecto-nucleotidase inhibitors." in: Biochemical pharmacology, Vol. 93, Issue 2, pp. 171-81, (2015) (PubMed).
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- ENPP2 (Ectonucleotide Pyrophosphatase / phosphodiesterase 2 (ENPP2))
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Alternative Name
- ENPP2
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Background
- The protein encoded by this gene functions as both a phosphodiesterase, which cleaves phosphodiester bonds at the 5' end of oligonucleotides, and a phospholipase, which catalyzes production of lysophosphatidic acid (LPA) in extracellular fluids. LPA evokes growth factor-like responses including stimulation of cell proliferation and chemotaxis. This gene product stimulates the motility of tumor cells and has angiogenic properties, and its expression is upregulated in several kinds of carcinomas. The gene product is secreted and further processed to make the biologically active form. Several alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Aug 2008].Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1, also known as alpha).
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NCBI Accession
- NM_006209, NP_006200
Target
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