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Human ETS2 cDNA Clone in Mammalian Expression Vector

This is a V-Ets Erythroblastosis Virus E26 Oncogene Homolog 2 plasmid from OriGene - 3 times cited - with cDNA insert cloned into Mammalian Expression VectorpCMV6-XL5. Insert length: 2560 bp. Transient expression. Suitable for PExp. Bacterial selection: Ampicillin.
OriGene
Catalog No. ABIN3383931
Supplier Product No.: sc116866
$452.43
Plus shipping costs $50.00
10 μg
Shipping to: United States
Delivery in 4 to 9 Business Days

Quick Overview for Human ETS2 cDNA Clone in Mammalian Expression Vector (ABIN3383931)

Gene

ETS2 (V-Ets Erythroblastosis Virus E26 Oncogene Homolog 2 (ETS2))

Application

Protein Expression (PExp)

Insert

cDNA

Vector

Mammalian Expression Vector

Vector Backbone

pCMV6-XL5

Promoter

Enhanced CMV Promoter, T7 Promoter

Bacterial Resistance

Ampicillin

Expression Type

Transient
  • Species

    Human

    Supplier Product No.

    sc116866

    Supplier

    OriGene

    Purpose

    Untagged full-length cDNA clone from Human ETS2 is ideal for over-expression of native protein for functional studies.

    Specificity

    Restriction Site: NotI-NotI

    Characteristics

    • These cDNA clones are isolated from full-length cDNA libraries and usually contain the coding sequence as well as the untranslated regions (UTRs) of the mRNA transcript appropriate to the library from which they were isolated.
    • These cDNA clones are ideal for over-expression of native proteins for functional studies. Provided as 10 μg transfection-ready plasmids.
    • Every lot of primer is tested to provide clean sequencing of cDNA clones.

    Purification

    The DNAs were purified using PowerPrep HP Plasmid isolation kits for transfection ready plasmids.

    Components

    • The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA.
    • The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

    Insert Length

    2560 bp

    Sequencing Primer

    VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3', XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
  • Restrictions

    For Research Use only
  • Format

    Lyophilized

    Storage

    RT,-20 °C

    Storage Comment

    The lyophilized plasmid is stable for up to one year when stored at ambient temperature. Following dissolution in 100 μL dH2O, store at -20 °C. Lyophilized primers are stable for up to one year when stored at ambient temperature. Following dissolution in 10 μL dH2O, store at -20 °C.

    Expiry Date

    12 months
  • Kabbout, Garcia, Fujimoto, Liu, Woods, Chow, Mendoza, Momin, James, Solis, Behrens, Lee, Wistuba, Kadara: "ETS2 mediated tumor suppressive function and MET oncogene inhibition in human non-small cell lung cancer." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 19, Issue 13, pp. 3383-95, (2013) (PubMed).

    Wang, Zeng, Luo, Jin, Chen: "Involvement of V-Ets erythroblastosis virus E26 oncogene homolog 2 in regulation of transcription activity of MDR1 gene." in: Acta biochimica et biophysica Sinica, Vol. 44, Issue 9, pp. 752-8, (2012) (PubMed).

    Cao, Littlejohn, Rodarte, Zhang, Martino, Rascoe, Hamid, Jupiter, Smythe: "Up-regulation of Bcl-xl by hepatocyte growth factor in human mesothelioma cells involves ETS transcription factors." in: The American journal of pathology, Vol. 175, Issue 5, pp. 2207-16, (2009) (PubMed).

  • Target

    ETS2 (V-Ets Erythroblastosis Virus E26 Oncogene Homolog 2 (ETS2))

    Alternative Name

    ETS2

    Background

    This gene encodes a transcription factor which regulates genes involved in development and apoptosis. The encoded protein is also a protooncogene and shown to be involved in regulation of telomerase. A pseudogene of this gene is located on the X chromosome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2012].Transcript Variant: This variant (1) represents the shorter transcript and encodes the shorter protein (isoform 1).

    NCBI Accession

    NM_005239, NP_005230
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