Human GABRA1 cDNA Clone in Mammalian Expression Vector
This is a gamma-aminobutyric Acid (GABA) A Receptor, alpha 1 plasmid from OriGene - 2 times cited - with cDNA insert cloned into Mammalian Expression VectorpCMV6-XL5. Insert length: 2290 bp. Transient expression. Suitable for PExp.
Bacterial selection: Ampicillin.
1 image
(2 references)
Catalog No. ABIN3384155
Supplier Product No.: sc119668
$679.14
Plus shipping costs $50.00
10 μg
Shipping to:
United States
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Quick Overview for Human GABRA1 cDNA Clone in Mammalian Expression Vector (ABIN3384155)
Gene
GABRA1
(gamma-aminobutyric Acid (GABA) A Receptor, alpha 1 (GABRA1))
Application
Protein Expression (PExp)
Insert
cDNA
Vector
Mammalian Expression Vector
Vector Backbone
pCMV6-XL5
Promoter
Enhanced CMV Promoter, T7 Promoter
Bacterial Resistance
Ampicillin
Expression Type
Transient
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Species
- Human
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Supplier Product No.
- sc119668
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Supplier
- OriGene
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Purpose
- Untagged full-length cDNA clone from Human GABRA1 is ideal for over-expression of native protein for functional studies.
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Specificity
- Restriction Site: NotI-NotI
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Characteristics
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- These cDNA clones are isolated from full-length cDNA libraries and usually contain the coding sequence as well as the untranslated regions (UTRs) of the mRNA transcript appropriate to the library from which they were isolated.
- These cDNA clones are ideal for over-expression of native proteins for functional studies. Provided as 10 μg transfection-ready plasmids.
- Every lot of primer is tested to provide clean sequencing of cDNA clones.
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Purification
- The DNAs were purified using PowerPrep HP Plasmid isolation kits for transfection ready plasmids.
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Components
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- The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA.
- The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
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Insert Length
- 2290 bp
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Sequencing Primer
- VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3', XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Storage
- RT,-20 °C
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Storage Comment
- The lyophilized plasmid is stable for up to one year when stored at ambient temperature. Following dissolution in 100 μL dH2O, store at -20 °C. Lyophilized primers are stable for up to one year when stored at ambient temperature. Following dissolution in 10 μL dH2O, store at -20 °C.
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Expiry Date
- 12 months
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: "Paraneoplastic Neurological Syndromes and Glutamic Acid Decarboxylase Antibodies." in: JAMA neurology, Vol. 72, Issue 8, pp. 874-81, (2015) (PubMed).
: "Multiple novel modes of action involved in the in vitro neurotoxic effects of tetrabromobisphenol-A." in: Toxicological sciences : an official journal of the Society of Toxicology, Vol. 128, Issue 1, pp. 235-46, (2012) (PubMed).
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: "Paraneoplastic Neurological Syndromes and Glutamic Acid Decarboxylase Antibodies." in: JAMA neurology, Vol. 72, Issue 8, pp. 874-81, (2015) (PubMed).
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- GABRA1 (gamma-aminobutyric Acid (GABA) A Receptor, alpha 1 (GABRA1))
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Alternative Name
- GABRA1
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Background
- This gene encodes a gamma-aminobutyric acid (GABA) receptor. GABA is the major inhibitory neurotransmitter in the mammalian brain where it acts at GABA-A receptors, which are ligand-gated chloride channels. Chloride conductance of these channels can be modulated by agents such as benzodiazepines that bind to the GABA-A receptor. GABA-A receptors are pentameric, consisting of proteins from several subunit classes: alpha, beta, gamma, delta and rho. Mutations in this gene cause juvenile myoclonic epilepsy and childhood absence epilepsy type 4. Multiple transcript variants encoding the same protein have been identified for this gene. [provided by RefSeq, Jul 2008].Transcript Variant: This variant (1) uses the 5'-most alternate exon in the 5' UTR. Variants 1-7 encode the same protein.
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NCBI Accession
- NM_000806, NP_000797
Target
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