Human GABRB3 cDNA Clone in Mammalian Expression Vector
This is a gamma-aminobutyric Acid (GABA) A Receptor, beta 3 plasmid from OriGene - 2 times cited - with cDNA insert cloned into Mammalian Expression VectorpCMV6-XL5. Insert length: 3080 bp. Transient expression. Suitable for PExp.
Bacterial selection: Ampicillin.
1 image
(2 references)
Catalog No. ABIN3384162
Supplier Product No.: sc125324
$452.43
Plus shipping costs $50.00
10 μg
Shipping to:
United States
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Quick Overview for Human GABRB3 cDNA Clone in Mammalian Expression Vector (ABIN3384162)
Gene
GABRB3
(gamma-aminobutyric Acid (GABA) A Receptor, beta 3 (GABRB3))
Application
Protein Expression (PExp)
Insert
cDNA
Vector
Mammalian Expression Vector
Vector Backbone
pCMV6-XL5
Promoter
Enhanced CMV Promoter, T7 Promoter
Bacterial Resistance
Ampicillin
Expression Type
Transient
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Species
- Human
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Supplier Product No.
- sc125324
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Supplier
- OriGene
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Purpose
- Untagged full-length cDNA clone from Human GABRB3 is ideal for over-expression of native protein for functional studies.
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Specificity
- Restriction Site: NotI-NotI
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Characteristics
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- These cDNA clones are isolated from full-length cDNA libraries and usually contain the coding sequence as well as the untranslated regions (UTRs) of the mRNA transcript appropriate to the library from which they were isolated.
- These cDNA clones are ideal for over-expression of native proteins for functional studies. Provided as 10 μg transfection-ready plasmids.
- Every lot of primer is tested to provide clean sequencing of cDNA clones.
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Purification
- The DNAs were purified using PowerPrep HP Plasmid isolation kits for transfection ready plasmids.
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Components
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- The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA.
- The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
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Insert Length
- 3080 bp
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Sequencing Primer
- VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3', XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Storage
- RT,-20 °C
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Storage Comment
- The lyophilized plasmid is stable for up to one year when stored at ambient temperature. Following dissolution in 100 μL dH2O, store at -20 °C. Lyophilized primers are stable for up to one year when stored at ambient temperature. Following dissolution in 10 μL dH2O, store at -20 °C.
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Expiry Date
- 12 months
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: "Paraneoplastic Neurological Syndromes and Glutamic Acid Decarboxylase Antibodies." in: JAMA neurology, Vol. 72, Issue 8, pp. 874-81, (2015) (PubMed).
: "Developmental modulation of GABA(A) receptor function by RNA editing." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 28, Issue 24, pp. 6196-201, (2008) (PubMed).
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: "Paraneoplastic Neurological Syndromes and Glutamic Acid Decarboxylase Antibodies." in: JAMA neurology, Vol. 72, Issue 8, pp. 874-81, (2015) (PubMed).
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- GABRB3 (gamma-aminobutyric Acid (GABA) A Receptor, beta 3 (GABRB3))
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Alternative Name
- GABRB3
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Background
- This gene encodes a member of the ligand-gated ionic channel family. The encoded protein is one the subunits of a multi-subunit chloride channel that serves as the receptor for gamma-aminobutyric acid, a major inhibitory neurotransmitter of the mammalian nervous system. This gene is located on the long arm of chromosome 15 in a cluster with two other genes encoding related subunits of the family. This gene may be associated with the pathogenesis of several disorders including Angelman syndrome, Prader-Willi syndrome, nonsyndromic orofacial clefts, epilepsy and autism. Alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2013].Transcript Variant: This variant (1) encodes isoform 1, which is of the same length as isoform 2, but has a distinct N-terminus.
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NCBI Accession
- NM_000814, NP_000805
Target
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