Human GADD45A cDNA Clone in Mammalian Expression Vector
This is a Growth Arrest and DNA-Damage-Inducible, alpha plasmid from OriGene - 3 times cited - with cDNA insert cloned into Mammalian Expression VectorpCMV6-XL5. Insert length: 1360 bp. Transient expression. Suitable for PExp.
Bacterial selection: Ampicillin.
1 image
(3 references)
Catalog No. ABIN3384168
Supplier Product No.: sc118947
$148.50
Plus shipping costs $50.00
10 μg
Shipping to:
United States
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Quick Overview for Human GADD45A cDNA Clone in Mammalian Expression Vector (ABIN3384168)
Gene
GADD45A
(Growth Arrest and DNA-Damage-Inducible, alpha (GADD45A))
Application
Protein Expression (PExp)
Insert
cDNA
Vector
Mammalian Expression Vector
Vector Backbone
pCMV6-XL5
Promoter
Enhanced CMV Promoter, T7 Promoter
Bacterial Resistance
Ampicillin
Expression Type
Transient
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Species
- Human
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Supplier Product No.
- sc118947
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Supplier
- OriGene
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Purpose
- Untagged full-length cDNA clone from Human GADD45A is ideal for over-expression of native protein for functional studies.
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Specificity
- Restriction Site: NotI-NotI
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Characteristics
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- These cDNA clones are isolated from full-length cDNA libraries and usually contain the coding sequence as well as the untranslated regions (UTRs) of the mRNA transcript appropriate to the library from which they were isolated.
- These cDNA clones are ideal for over-expression of native proteins for functional studies. Provided as 10 μg transfection-ready plasmids.
- Every lot of primer is tested to provide clean sequencing of cDNA clones.
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Purification
- The DNAs were purified using PowerPrep HP Plasmid isolation kits for transfection ready plasmids.
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Components
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- The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA.
- The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
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Insert Length
- 1360 bp
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Sequencing Primer
- VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3', XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Storage
- RT,-20 °C
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Storage Comment
- The lyophilized plasmid is stable for up to one year when stored at ambient temperature. Following dissolution in 100 μL dH2O, store at -20 °C. Lyophilized primers are stable for up to one year when stored at ambient temperature. Following dissolution in 10 μL dH2O, store at -20 °C.
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Expiry Date
- 12 months
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: "CArG-driven GADD45α activated by resveratrol inhibits lung cancer cells." in: Genes & cancer, Vol. 6, Issue 5-6, pp. 220-30, (2015) (PubMed).
: "p53 controls hepatitis C virus non-structural protein 5A-mediated downregulation of GADD45α expression via the NF-κB and PI3K-Akt pathways." in: The Journal of general virology, Vol. 94, Issue Pt 2, pp. 326-35, (2013) (PubMed).
: "Methylation-mediated repression of GADD45alpha in prostate cancer and its role as a potential therapeutic target." in: Cancer research, Vol. 69, Issue 4, pp. 1527-35, (2009) (PubMed).
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: "CArG-driven GADD45α activated by resveratrol inhibits lung cancer cells." in: Genes & cancer, Vol. 6, Issue 5-6, pp. 220-30, (2015) (PubMed).
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- GADD45A (Growth Arrest and DNA-Damage-Inducible, alpha (GADD45A))
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Alternative Name
- GADD45A
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Background
- This gene is a member of a group of genes whose transcript levels are increased following stressful growth arrest conditions and treatment with DNA-damaging agents. The protein encoded by this gene responds to environmental stresses by mediating activation of the p38/JNK pathway via MTK1/MEKK4 kinase. The DNA damage-induced transcription of this gene is mediated by both p53-dependent and -independent mechanisms. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.[provided by RefSeq, Dec 2010].Transcript Variant: This variant (1) encodes the longest isoform (1).
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NCBI Accession
- NM_001924, NP_001915
Target
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