Human HBB cDNA Clone in Mammalian Expression Vector
This is a Hemoglobin beta plasmid from OriGene - one-time cited - with cDNA insert cloned into Mammalian Expression VectorpCMV6-XL5. Insert length: 740 bp. Transient expression. Suitable for PExp.
Bacterial selection: Ampicillin.
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Catalog No. ABIN3384377
Supplier Product No.: sc119811
$222.75
Plus shipping costs $50.00
10 μg
Shipping to:
United States
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Quick Overview for Human HBB cDNA Clone in Mammalian Expression Vector (ABIN3384377)
Gene
Hemoglobin Subunit beta (HBB)
(Hemoglobin beta (HBB))
Application
Protein Expression (PExp)
Insert
cDNA
Vector
Mammalian Expression Vector
Vector Backbone
pCMV6-XL5
Promoter
Enhanced CMV Promoter, T7 Promoter
Bacterial Resistance
Ampicillin
Expression Type
Transient
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Species
- Human
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Supplier Product No.
- sc119811
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Supplier
- OriGene
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Purpose
- Untagged full-length cDNA clone from Human HBB is ideal for over-expression of native protein for functional studies.
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Specificity
- Restriction Site: NotI-NotI
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Characteristics
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- These cDNA clones are isolated from full-length cDNA libraries and usually contain the coding sequence as well as the untranslated regions (UTRs) of the mRNA transcript appropriate to the library from which they were isolated.
- These cDNA clones are ideal for over-expression of native proteins for functional studies. Provided as 10 μg transfection-ready plasmids.
- Every lot of primer is tested to provide clean sequencing of cDNA clones.
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Purification
- The DNAs were purified using PowerPrep HP Plasmid isolation kits for transfection ready plasmids.
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Components
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- The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA.
- The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
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Insert Length
- 740 bp
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Sequencing Primer
- VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3', XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Storage
- RT,-20 °C
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Storage Comment
- The lyophilized plasmid is stable for up to one year when stored at ambient temperature. Following dissolution in 100 μL dH2O, store at -20 °C. Lyophilized primers are stable for up to one year when stored at ambient temperature. Following dissolution in 10 μL dH2O, store at -20 °C.
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Expiry Date
- 12 months
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: "Determinants of specific RNA interference-mediated silencing of human beta-globin alleles differing by a single nucleotide polymorphism." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 103, Issue 15, pp. 5953-8, (2006) (PubMed).
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: "Determinants of specific RNA interference-mediated silencing of human beta-globin alleles differing by a single nucleotide polymorphism." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 103, Issue 15, pp. 5953-8, (2006) (PubMed).
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- Hemoglobin Subunit beta (HBB) (Hemoglobin beta (HBB))
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Alternative Name
- HBB
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Target Type
- Viral Protein
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Background
- The alpha (HBA) and beta (HBB) loci determine the structure of the 2 types of polypeptide chains in adult hemoglobin, Hb A. The normal adult hemoglobin tetramer consists of two alpha chains and two beta chains. Mutant beta globin causes sickle cell anemia. Absence of beta chain causes beta-zero-thalassemia. Reduced amounts of detectable beta globin causes beta-plus-thalassemia. The order of the genes in the beta-globin cluster is 5'-epsilon -- gamma-G -- gamma-A -- delta -- beta--3'. [provided by RefSeq, Jul 2008].
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NCBI Accession
- NM_000518, NP_000509
Target
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