Human HDAC1 cDNA Clone in Mammalian Expression Vector
This is a Histone Deacetylase 1 plasmid from OriGene - 4 times cited - with cDNA insert cloned into Mammalian Expression VectorpCMV6-XL5. Insert length: 2200 bp. Transient expression. Suitable for PExp.
Bacterial selection: Ampicillin.
1 image
(4 references)
Catalog No. ABIN3384386
Supplier Product No.: sc117054
$679.14
Plus shipping costs $50.00
10 μg
Shipping to:
United States
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Quick Overview for Human HDAC1 cDNA Clone in Mammalian Expression Vector (ABIN3384386)
Gene
HDAC1
(Histone Deacetylase 1 (HDAC1))
Application
Protein Expression (PExp)
Insert
cDNA
Vector
Mammalian Expression Vector
Vector Backbone
pCMV6-XL5
Promoter
Enhanced CMV Promoter, T7 Promoter
Bacterial Resistance
Ampicillin
Expression Type
Transient
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Species
- Human
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Supplier Product No.
- sc117054
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Supplier
- OriGene
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Purpose
- Untagged full-length cDNA clone from Human HDAC1 is ideal for over-expression of native protein for functional studies.
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Specificity
- Restriction Site: NotI-NotI
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Characteristics
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- These cDNA clones are isolated from full-length cDNA libraries and usually contain the coding sequence as well as the untranslated regions (UTRs) of the mRNA transcript appropriate to the library from which they were isolated.
- These cDNA clones are ideal for over-expression of native proteins for functional studies. Provided as 10 μg transfection-ready plasmids.
- Every lot of primer is tested to provide clean sequencing of cDNA clones.
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Purification
- The DNAs were purified using PowerPrep HP Plasmid isolation kits for transfection ready plasmids.
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Components
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- The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA.
- The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
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Insert Length
- 2200 bp
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Sequencing Primer
- VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3', XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Storage
- RT,-20 °C
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Storage Comment
- The lyophilized plasmid is stable for up to one year when stored at ambient temperature. Following dissolution in 100 μL dH2O, store at -20 °C. Lyophilized primers are stable for up to one year when stored at ambient temperature. Following dissolution in 10 μL dH2O, store at -20 °C.
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Expiry Date
- 12 months
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: "AIRE acetylation and deacetylation: effect on protein stability and transactivation activity." in: Journal of biomedical science, Vol. 21, pp. 85, (2014) (PubMed).
: "Stimulation of histone deacetylase activity by metabolites of intermediary metabolism." in: The Journal of biological chemistry, Vol. 287, Issue 38, pp. 32006-16, (2012) (PubMed).
: "A novel domain in histone deacetylase 1 and 2 mediates repression of cartilage-specific genes in human chondrocytes." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 23, Issue 10, pp. 3539-52, (2009) (PubMed).
: "Identification of cellular proteins that maintain retroviral epigenetic silencing: evidence for an antiviral response." in: Journal of virology, Vol. 82, Issue 5, pp. 2313-23, (2008) (PubMed).
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: "AIRE acetylation and deacetylation: effect on protein stability and transactivation activity." in: Journal of biomedical science, Vol. 21, pp. 85, (2014) (PubMed).
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- HDAC1 (Histone Deacetylase 1 (HDAC1))
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Alternative Name
- HDAC1
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Background
- Histone acetylation and deacetylation, catalyzed by multisubunit complexes, play a key role in the regulation of eukaryotic gene expression. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex. It also interacts with retinoblastoma tumor-suppressor protein and this complex is a key element in the control of cell proliferation and differentiation. Together with metastasis-associated protein-2, it deacetylates p53 and modulates its effect on cell growth and apoptosis. [provided by RefSeq, Jul 2008].
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NCBI Accession
- NM_004964, NP_004955
Target
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