Human HMGN3 cDNA Clone in Mammalian Expression Vector
This is a High Mobility Group Nucleosomal Binding Domain 3 plasmid from OriGene - one-time cited - with cDNA insert cloned into Mammalian Expression VectorpCMV6-XL5. Insert length: 800 bp. Transient expression. Suitable for PExp.
Bacterial selection: Ampicillin.
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(1 reference)
Catalog No. ABIN3384462
Supplier Product No.: sc110044
$148.50
Plus shipping costs $50.00
10 μg
Shipping to:
United States
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Quick Overview for Human HMGN3 cDNA Clone in Mammalian Expression Vector (ABIN3384462)
Gene
HMGN3
(High Mobility Group Nucleosomal Binding Domain 3 (HMGN3))
Application
Protein Expression (PExp)
Insert
cDNA
Vector
Mammalian Expression Vector
Vector Backbone
pCMV6-XL5
Promoter
Enhanced CMV Promoter, T7 Promoter
Bacterial Resistance
Ampicillin
Expression Type
Transient
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Species
- Human
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Supplier Product No.
- sc110044
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Supplier
- OriGene
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Purpose
- Untagged full-length cDNA clone from Human HMGN3 is ideal for over-expression of native protein for functional studies.
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Specificity
- Restriction Site: NotI-NotI
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Characteristics
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- These cDNA clones are isolated from full-length cDNA libraries and usually contain the coding sequence as well as the untranslated regions (UTRs) of the mRNA transcript appropriate to the library from which they were isolated.
- These cDNA clones are ideal for over-expression of native proteins for functional studies. Provided as 10 μg transfection-ready plasmids.
- Every lot of primer is tested to provide clean sequencing of cDNA clones.
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Purification
- The DNAs were purified using PowerPrep HP Plasmid isolation kits for transfection ready plasmids.
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Components
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- The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA.
- The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
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Insert Length
- 800 bp
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Sequencing Primer
- VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3', XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Storage
- RT,-20 °C
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Storage Comment
- The lyophilized plasmid is stable for up to one year when stored at ambient temperature. Following dissolution in 100 μL dH2O, store at -20 °C. Lyophilized primers are stable for up to one year when stored at ambient temperature. Following dissolution in 10 μL dH2O, store at -20 °C.
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Expiry Date
- 12 months
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: "ZBTB32 is an early repressor of the CIITA and MHC class II gene expression during B cell differentiation to plasma cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 189, Issue 5, pp. 2393-403, (2012) (PubMed).
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: "ZBTB32 is an early repressor of the CIITA and MHC class II gene expression during B cell differentiation to plasma cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 189, Issue 5, pp. 2393-403, (2012) (PubMed).
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- HMGN3 (High Mobility Group Nucleosomal Binding Domain 3 (HMGN3))
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Alternative Name
- HMGN3
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Background
- The protein encoded by this gene binds thyroid hormone receptor beta in the presence of thyroid hormone. The encoded protein, a member of the HMGN protein family, is thought to reduce the compactness of the chromatin fiber in nucleosomes, thereby enhancing transcription from chromatin templates. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. There is a related pseudogene on chromosome 1. [provided by RefSeq, Jan 2016].Transcript Variant: This variant (2) lacks an alternate segment in the 3' coding region compared to variant 1, which causes a frame-shift. The resulting isoform (HMGN3b) is shorter and has a distinct C-terminus compared to isoform HMGN3a.
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NCBI Accession
- NM_138730, NP_620058
Target
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