Human HSPD1 cDNA Clone in Mammalian Expression Vector
This is a Heat Shock 60kDa Protein 1 (Chaperonin) plasmid from OriGene - one-time cited - with cDNA insert cloned into Mammalian Expression VectorpCMV6-XL5. Insert length: 2240 bp. Transient expression. Suitable for PExp.
Bacterial selection: Ampicillin.
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(1 reference)
Catalog No. ABIN3384548
Supplier Product No.: sc111640
$528.66
Plus shipping costs $50.00
10 μg
Shipping to:
United States
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Quick Overview for Human HSPD1 cDNA Clone in Mammalian Expression Vector (ABIN3384548)
Gene
HSPD1
(Heat Shock 60kDa Protein 1 (Chaperonin) (HSPD1))
Application
Protein Expression (PExp)
Insert
cDNA
Vector
Mammalian Expression Vector
Vector Backbone
pCMV6-XL5
Promoter
Enhanced CMV Promoter, T7 Promoter
Bacterial Resistance
Ampicillin
Expression Type
Transient
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Species
- Human
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Supplier Product No.
- sc111640
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Supplier
- OriGene
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Purpose
- Untagged full-length cDNA clone from Human HSPD1 is ideal for over-expression of native protein for functional studies.
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Specificity
- Restriction Site: NotI-NotI
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Characteristics
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- These cDNA clones are isolated from full-length cDNA libraries and usually contain the coding sequence as well as the untranslated regions (UTRs) of the mRNA transcript appropriate to the library from which they were isolated.
- These cDNA clones are ideal for over-expression of native proteins for functional studies. Provided as 10 μg transfection-ready plasmids.
- Every lot of primer is tested to provide clean sequencing of cDNA clones.
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Purification
- The DNAs were purified using PowerPrep HP Plasmid isolation kits for transfection ready plasmids.
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Components
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- The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA.
- The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
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Insert Length
- 2240 bp
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Sequencing Primer
- VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3', XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Storage
- RT,-20 °C
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Storage Comment
- The lyophilized plasmid is stable for up to one year when stored at ambient temperature. Following dissolution in 100 μL dH2O, store at -20 °C. Lyophilized primers are stable for up to one year when stored at ambient temperature. Following dissolution in 10 μL dH2O, store at -20 °C.
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Expiry Date
- 12 months
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: "Oxidant-induced apoptosis is mediated by oxidation of the actin-regulatory protein cofilin." in: Nature cell biology, Vol. 11, Issue 10, pp. 1241-6, (2009) (PubMed).
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: "Oxidant-induced apoptosis is mediated by oxidation of the actin-regulatory protein cofilin." in: Nature cell biology, Vol. 11, Issue 10, pp. 1241-6, (2009) (PubMed).
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- HSPD1 (Heat Shock 60kDa Protein 1 (Chaperonin) (HSPD1))
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Alternative Name
- HSPD1
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Background
- This gene encodes a member of the chaperonin family. The encoded mitochondrial protein may function as a signaling molecule in the innate immune system. This protein is essential for the folding and assembly of newly imported proteins in the mitochondria. This gene is adjacent to a related family member and the region between the 2 genes functions as a bidirectional promoter. Several pseudogenes have been associated with this gene. Two transcript variants encoding the same protein have been identified for this gene. Mutations associated with this gene cause autosomal recessive spastic paraplegia 13. [provided by RefSeq, Jun 2010].Transcript Variant: This variant (1) represents the longer transcript. Both variants 1 and 2 encode the same protein.
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NCBI Accession
- NM_002156, NP_002147
Target
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