Human HTR2A cDNA Clone in Mammalian Expression Vector
This is a 5-Hydroxytryptamine (serotonin) Receptor 2A plasmid from OriGene - 2 times cited - with cDNA insert cloned into Mammalian Expression VectorpCMV6-XL5. Insert length: 3300 bp. Transient expression. Suitable for PExp.
Bacterial selection: Ampicillin.
1 image
(2 references)
Catalog No. ABIN3384552
Supplier Product No.: sc119793
$679.14
Plus shipping costs $50.00
10 μg
Shipping to:
United States
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Quick Overview for Human HTR2A cDNA Clone in Mammalian Expression Vector (ABIN3384552)
Gene
HTR2A
(5-Hydroxytryptamine (serotonin) Receptor 2A (HTR2A))
Application
Protein Expression (PExp)
Insert
cDNA
Vector
Mammalian Expression Vector
Vector Backbone
pCMV6-XL5
Promoter
Enhanced CMV Promoter, T7 Promoter
Bacterial Resistance
Ampicillin
Expression Type
Transient
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Species
- Human
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Supplier Product No.
- sc119793
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Supplier
- OriGene
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Purpose
- Untagged full-length cDNA clone from Human HTR2A is ideal for over-expression of native protein for functional studies.
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Specificity
- Restriction Site: NotI-NotI
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Characteristics
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- These cDNA clones are isolated from full-length cDNA libraries and usually contain the coding sequence as well as the untranslated regions (UTRs) of the mRNA transcript appropriate to the library from which they were isolated.
- These cDNA clones are ideal for over-expression of native proteins for functional studies. Provided as 10 μg transfection-ready plasmids.
- Every lot of primer is tested to provide clean sequencing of cDNA clones.
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Purification
- The DNAs were purified using PowerPrep HP Plasmid isolation kits for transfection ready plasmids.
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Components
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- The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA.
- The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
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Insert Length
- 3300 bp
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Sequencing Primer
- VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3', XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Storage
- RT,-20 °C
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Storage Comment
- The lyophilized plasmid is stable for up to one year when stored at ambient temperature. Following dissolution in 100 μL dH2O, store at -20 °C. Lyophilized primers are stable for up to one year when stored at ambient temperature. Following dissolution in 10 μL dH2O, store at -20 °C.
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Expiry Date
- 12 months
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: "Molecular pharmacology and ligand docking studies reveal a single amino acid difference between mouse and human serotonin 5-HT2A receptors that impacts behavioral translation of novel ..." in: The Journal of pharmacology and experimental therapeutics, Vol. 347, Issue 3, pp. 705-16, (2013) (PubMed).
: "c-Src tyrosine kinase, a critical component for 5-HT2A receptor-mediated contraction in rat aorta." in: The Journal of physiology, Vol. 586, Issue 16, pp. 3855-69, (2008) (PubMed).
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: "Molecular pharmacology and ligand docking studies reveal a single amino acid difference between mouse and human serotonin 5-HT2A receptors that impacts behavioral translation of novel ..." in: The Journal of pharmacology and experimental therapeutics, Vol. 347, Issue 3, pp. 705-16, (2013) (PubMed).
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- HTR2A (5-Hydroxytryptamine (serotonin) Receptor 2A (HTR2A))
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Alternative Name
- HTR2A
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Background
- This gene encodes one of the receptors for serotonin, a neurotransmitter with many roles. Mutations in this gene are associated with susceptibility to schizophrenia and obsessive-compulsive disorder, and are also associated with response to the antidepressant citalopram in patients with major depressive disorder (MDD). MDD patients who also have a mutation in intron 2 of this gene show a significantly reduced response to citalopram as this antidepressant downregulates expression of this gene. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009].Transcript Variant: This variant (1) represents the longer transcript and encodes the longer protein (isoform 1).
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NCBI Accession
- NM_000621, NP_000612
Target
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