Human IRF1 cDNA Clone in Mammalian Expression Vector
This is a Interferon Regulatory Factor 1 plasmid from OriGene - 4 times cited - with cDNA insert cloned into Mammalian Expression VectorpCMV6-XL5. Insert length: 2600 bp. Transient expression. Suitable for PExp.
Bacterial selection: Ampicillin.
1 image
(4 references)
Catalog No. ABIN3384654
Supplier Product No.: sc118744
$445.50
Plus shipping costs $50.00
10 μg
Shipping to:
United States
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Quick Overview for Human IRF1 cDNA Clone in Mammalian Expression Vector (ABIN3384654)
Gene
IRF1
(Interferon Regulatory Factor 1 (IRF1))
Application
Protein Expression (PExp)
Insert
cDNA
Vector
Mammalian Expression Vector
Vector Backbone
pCMV6-XL5
Promoter
Enhanced CMV Promoter, T7 Promoter
Bacterial Resistance
Ampicillin
Expression Type
Transient
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Species
- Human
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Supplier Product No.
- sc118744
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Supplier
- OriGene
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Purpose
- Untagged full-length cDNA clone from Human IRF1 is ideal for over-expression of native protein for functional studies.
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Specificity
- Restriction Site: NotI-NotI
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Characteristics
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- These cDNA clones are isolated from full-length cDNA libraries and usually contain the coding sequence as well as the untranslated regions (UTRs) of the mRNA transcript appropriate to the library from which they were isolated.
- These cDNA clones are ideal for over-expression of native proteins for functional studies. Provided as 10 μg transfection-ready plasmids.
- Every lot of primer is tested to provide clean sequencing of cDNA clones.
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Purification
- The DNAs were purified using PowerPrep HP Plasmid isolation kits for transfection ready plasmids.
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Components
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- The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA.
- The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
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Insert Length
- 2600 bp
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Sequencing Primer
- VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3', XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Storage
- RT,-20 °C
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Storage Comment
- The lyophilized plasmid is stable for up to one year when stored at ambient temperature. Following dissolution in 100 μL dH2O, store at -20 °C. Lyophilized primers are stable for up to one year when stored at ambient temperature. Following dissolution in 10 μL dH2O, store at -20 °C.
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Expiry Date
- 12 months
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: "Dysregulation of interferon regulatory factors impairs the expression of immunostimulatory molecules in hepatitis C virus genotype 1-infected hepatocytes." in: Gut, Vol. 63, Issue 4, pp. 665-73, (2014) (PubMed).
: "Immune mediators regulate CFTR expression through a bifunctional airway-selective enhancer." in: Molecular and cellular biology, Vol. 33, Issue 15, pp. 2843-53, (2013) (PubMed).
: "Virus-activated interferon regulatory factor 7 upregulates expression of the interferon-regulated BST2 gene independently of interferon signaling." in: Journal of virology, Vol. 86, Issue 7, pp. 3513-27, (2012) (PubMed).
: "IRF-1 and miRNA126 modulate VCAM-1 expression in response to a high-fat meal." in: Circulation research, Vol. 111, Issue 8, pp. 1054-64, (2012) (PubMed).
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- IRF1 (Interferon Regulatory Factor 1 (IRF1))
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Alternative Name
- IRF1
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Background
- IRF1 encodes interferon regulatory factor 1, a member of the interferon regulatory transcription factor (IRF) family. IRF1 serves as an activator of interferons alpha and beta transcription, and in mouse it has been shown to be required for double-stranded RNA induction of these genes. IRF1 also functions as a transcription activator of genes induced by interferons alpha, beta, and gamma. Further, IRF1 has been shown to play roles in regulating apoptosis and tumor-suppressoion. [provided by RefSeq, Jul 2008].
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NCBI Accession
- NM_002198, NP_002189
Target
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