Human ITGB1 cDNA Clone in Mammalian Expression Vector
This is a Integrin beta 1 plasmid from OriGene - 3 times cited - with cDNA insert cloned into Mammalian Expression VectorpCMV6-XL5. Insert length: 3800 bp. Transient expression. Suitable for PExp.
Bacterial selection: Ampicillin.
1 image
(3 references)
Catalog No. ABIN3384669
Supplier Product No.: sc111935
$1,086.03
Plus shipping costs $50.00
10 μg
Shipping to:
United States
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Quick Overview for Human ITGB1 cDNA Clone in Mammalian Expression Vector (ABIN3384669)
Gene
ITGB1
(Integrin beta 1 (ITGB1))
Application
Protein Expression (PExp)
Insert
cDNA
Vector
Mammalian Expression Vector
Vector Backbone
pCMV6-XL5
Promoter
Enhanced CMV Promoter, T7 Promoter
Bacterial Resistance
Ampicillin
Expression Type
Transient
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Species
- Human
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Supplier Product No.
- sc111935
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Supplier
- OriGene
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Purpose
- Untagged full-length cDNA clone from Human ITGB1 is ideal for over-expression of native protein for functional studies.
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Specificity
- Restriction Site: NotI-NotI
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Characteristics
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- These cDNA clones are isolated from full-length cDNA libraries and usually contain the coding sequence as well as the untranslated regions (UTRs) of the mRNA transcript appropriate to the library from which they were isolated.
- These cDNA clones are ideal for over-expression of native proteins for functional studies. Provided as 10 μg transfection-ready plasmids.
- Every lot of primer is tested to provide clean sequencing of cDNA clones.
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Purification
- The DNAs were purified using PowerPrep HP Plasmid isolation kits for transfection ready plasmids.
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Components
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- The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA.
- The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
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Insert Length
- 3800 bp
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Sequencing Primer
- VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3', XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Storage
- RT,-20 °C
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Storage Comment
- The lyophilized plasmid is stable for up to one year when stored at ambient temperature. Following dissolution in 100 μL dH2O, store at -20 °C. Lyophilized primers are stable for up to one year when stored at ambient temperature. Following dissolution in 10 μL dH2O, store at -20 °C.
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Expiry Date
- 12 months
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: "Twist induces epithelial-mesenchymal transition and cell motility in breast cancer via ITGB1-FAK/ILK signaling axis and its associated downstream network." in: The international journal of biochemistry & cell biology, Vol. 71, pp. 62-71, (2016) (PubMed).
: "Gln-362 of angiopoietin-2 mediates migration of tumor and endothelial cells through association with α5β1 integrin." in: The Journal of biological chemistry, Vol. 289, Issue 45, pp. 31330-40, (2014) (PubMed).
: "SNX17 protects integrins from degradation by sorting between lysosomal and recycling pathways." in: The Journal of cell biology, Vol. 197, Issue 2, pp. 219-30, (2012) (PubMed).
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- ITGB1 (Integrin beta 1 (ITGB1))
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Alternative Name
- ITGB1
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Background
- Integrins are heterodimeric proteins made up of alpha and beta subunits. At least 18 alpha and 8 beta subunits have been described in mammals. Integrin family members are membrane receptors involved in cell adhesion and recognition in a variety of processes including embryogenesis, hemostasis, tissue repair, immune response and metastatic diffusion of tumor cells. This gene encodes a beta subunit. Multiple alternatively spliced transcript variants which encode different protein isoforms have been found for this gene. [provided by RefSeq, Jul 2008].Transcript Variant: This variant (1A) represents the longest transcript and encodes the shorter isoform (1A). Both variants 1A and 1E encode the same isoform.
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NCBI Accession
- NM_002211, NP_002202
Target
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