Human KCND2 cDNA Clone in Mammalian Expression Vector
This is a Potassium Voltage-Gated Channel, Shal-Related Subfamily, Member 2 plasmid from OriGene - 3 times cited - with cDNA insert cloned into Mammalian Expression VectorpCMV6-XL5. Insert length: 4600 bp. Transient expression. Suitable for PExp.
Bacterial selection: Ampicillin.
1 image
(3 references)
Catalog No. ABIN3384709
Supplier Product No.: sc115436
$581.13
Plus shipping costs $50.00
10 μg
Shipping to:
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Quick Overview for Human KCND2 cDNA Clone in Mammalian Expression Vector (ABIN3384709)
Gene
KCND2
(Potassium Voltage-Gated Channel, Shal-Related Subfamily, Member 2 (KCND2))
Application
Protein Expression (PExp)
Insert
cDNA
Vector
Mammalian Expression Vector
Vector Backbone
pCMV6-XL5
Promoter
Enhanced CMV Promoter, T7 Promoter
Bacterial Resistance
Ampicillin
Expression Type
Transient
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Species
- Human
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Supplier Product No.
- sc115436
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Supplier
- OriGene
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Purpose
- Untagged full-length cDNA clone from Human KCND2 is ideal for over-expression of native protein for functional studies.
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Specificity
- Restriction Site: NotI-NotI
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Characteristics
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- These cDNA clones are isolated from full-length cDNA libraries and usually contain the coding sequence as well as the untranslated regions (UTRs) of the mRNA transcript appropriate to the library from which they were isolated.
- These cDNA clones are ideal for over-expression of native proteins for functional studies. Provided as 10 μg transfection-ready plasmids.
- Every lot of primer is tested to provide clean sequencing of cDNA clones.
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Purification
- The DNAs were purified using PowerPrep HP Plasmid isolation kits for transfection ready plasmids.
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Components
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- The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA.
- The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
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Insert Length
- 4600 bp
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Sequencing Primer
- VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3', XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Storage
- RT,-20 °C
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Storage Comment
- The lyophilized plasmid is stable for up to one year when stored at ambient temperature. Following dissolution in 100 μL dH2O, store at -20 °C. Lyophilized primers are stable for up to one year when stored at ambient temperature. Following dissolution in 10 μL dH2O, store at -20 °C.
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Expiry Date
- 12 months
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: "Regulation of neuronal activity by Cav3-Kv4 channel signaling complexes." in: Nature neuroscience, Vol. 13, Issue 3, pp. 333-7, (2010) (PubMed).
: "Traffic of Kv4 K+ channels mediated by KChIP1 is via a novel post-ER vesicular pathway." in: The Journal of cell biology, Vol. 171, Issue 3, pp. 459-69, (2005) (PubMed).
: "Murine glycosyltransferases responsible for the expression of globo-series glycolipids: cDNA structures, mRNA expression, and distribution of their products." in: Glycobiology, Vol. 15, Issue 12, pp. 1257-67, (2005) (PubMed).
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: "Regulation of neuronal activity by Cav3-Kv4 channel signaling complexes." in: Nature neuroscience, Vol. 13, Issue 3, pp. 333-7, (2010) (PubMed).
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- KCND2 (Potassium Voltage-Gated Channel, Shal-Related Subfamily, Member 2 (KCND2))
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Alternative Name
- KCND2
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Background
- Voltage-gated potassium (Kv) channels represent the most complex class of voltage-gated ion channels from both functional and structural standpoints. Their diverse functions include regulating neurotransmitter release, heart rate, insulin secretion, neuronal excitability, epithelial electrolyte transport, smooth muscle contraction, and cell volume. Four sequence-related potassium channel genes - shaker, shaw, shab, and shal - have been identified in Drosophila, and each has been shown to have human homolog(s). This gene encodes a member of the potassium channel, voltage-gated, shal-related subfamily, members of which form voltage-activated A-type potassium ion channels and are prominent in the repolarization phase of the action potential. This member mediates a rapidly inactivating, A-type outward potassium current which is not under the control of the N terminus as it is in Shaker channels. [provided by RefSeq, Jul 2008].
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NCBI Accession
- NM_012281, NP_036413
Target
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