Human MGMT cDNA Clone in Mammalian Expression Vector
This is a O6-Methylguanine-DNA-Methyltransferase plasmid from OriGene - 2 times cited - with cDNA insert cloned into Mammalian Expression VectorpCMV6-XL5. Insert length: 870 bp. Transient expression. Suitable for PExp.
Bacterial selection: Ampicillin.
1 image
(2 references)
Catalog No. ABIN3385133
Supplier Product No.: sc108494
$445.50
Plus shipping costs $50.00
10 μg
Shipping to:
United States
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Quick Overview for Human MGMT cDNA Clone in Mammalian Expression Vector (ABIN3385133)
Gene
MGMT
(O6-Methylguanine-DNA-Methyltransferase (MGMT))
Application
Protein Expression (PExp)
Insert
cDNA
Vector
Mammalian Expression Vector
Vector Backbone
pCMV6-XL5
Promoter
Enhanced CMV Promoter, T7 Promoter
Bacterial Resistance
Ampicillin
Expression Type
Transient
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Species
- Human
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Supplier Product No.
- sc108494
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Supplier
- OriGene
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Purpose
- Untagged full-length cDNA clone from Human MGMT is ideal for over-expression of native protein for functional studies.
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Specificity
- Restriction Site: NotI-NotI
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Characteristics
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- These cDNA clones are isolated from full-length cDNA libraries and usually contain the coding sequence as well as the untranslated regions (UTRs) of the mRNA transcript appropriate to the library from which they were isolated.
- These cDNA clones are ideal for over-expression of native proteins for functional studies. Provided as 10 μg transfection-ready plasmids.
- Every lot of primer is tested to provide clean sequencing of cDNA clones.
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Purification
- The DNAs were purified using PowerPrep HP Plasmid isolation kits for transfection ready plasmids.
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Components
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- The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA.
- The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
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Insert Length
- 870 bp
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Sequencing Primer
- VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3', XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Storage
- RT,-20 °C
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Storage Comment
- The lyophilized plasmid is stable for up to one year when stored at ambient temperature. Following dissolution in 100 μL dH2O, store at -20 °C. Lyophilized primers are stable for up to one year when stored at ambient temperature. Following dissolution in 10 μL dH2O, store at -20 °C.
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Expiry Date
- 12 months
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: "Profound prevention of experimental brain metastases of breast cancer by temozolomide in an MGMT-dependent manner." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 20, Issue 10, pp. 2727-39, (2014) (PubMed).
: "miR-181d: a predictive glioblastoma biomarker that downregulates MGMT expression." in: Neuro-oncology, Vol. 14, Issue 6, pp. 712-9, (2012) (PubMed).
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- MGMT (O6-Methylguanine-DNA-Methyltransferase (MGMT))
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Alternative Name
- MGMT
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Background
- Alkylating agents are potent carcinogens that can result in cell death, mutation and cancer. The protein encoded by this gene is a DNA repair protein that is involved in cellular defense against mutagenesis and toxicity from alkylating agents. The protein catalyzes transfer of methyl groups from O(6)-alkylguanine and other methylated moieties of the DNA to its own molecule, which repairs the toxic lesions. Methylation of the genes promoter has been associated with several cancer types, including colorectal cancer, lung cancer, lymphoma and glioblastoma. [provided by RefSeq, Sep 2015].
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NCBI Accession
- NM_002412, NP_002403
Target
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