Human MTF1 cDNA Clone in Mammalian Expression Vector
This is a Metal-Regulatory Transcription Factor 1 plasmid from OriGene - 2 times cited - with cDNA insert cloned into Mammalian Expression VectorpCMV6-XL5. Insert length: 2980 bp. Transient expression. Suitable for PExp.
Bacterial selection: Ampicillin.
1 image
(2 references)
Catalog No. ABIN3385278
Supplier Product No.: sc116396
$683.10
Plus shipping costs $50.00
10 μg
Shipping to:
United States
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Quick Overview for Human MTF1 cDNA Clone in Mammalian Expression Vector (ABIN3385278)
Gene
MTF1
(Metal-Regulatory Transcription Factor 1 (MTF1))
Application
Protein Expression (PExp)
Insert
cDNA
Vector
Mammalian Expression Vector
Vector Backbone
pCMV6-XL5
Promoter
Enhanced CMV Promoter, T7 Promoter
Bacterial Resistance
Ampicillin
Expression Type
Transient
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Species
- Human
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Supplier Product No.
- sc116396
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Supplier
- OriGene
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Purpose
- Untagged full-length cDNA clone from Human MTF1 is ideal for over-expression of native protein for functional studies.
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Specificity
- Restriction Site: NotI-NotI
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Characteristics
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- These cDNA clones are isolated from full-length cDNA libraries and usually contain the coding sequence as well as the untranslated regions (UTRs) of the mRNA transcript appropriate to the library from which they were isolated.
- These cDNA clones are ideal for over-expression of native proteins for functional studies. Provided as 10 μg transfection-ready plasmids.
- Every lot of primer is tested to provide clean sequencing of cDNA clones.
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Purification
- The DNAs were purified using PowerPrep HP Plasmid isolation kits for transfection ready plasmids.
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Components
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- The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA.
- The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
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Insert Length
- 2980 bp
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Sequencing Primer
- VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3', XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Storage
- RT,-20 °C
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Storage Comment
- The lyophilized plasmid is stable for up to one year when stored at ambient temperature. Following dissolution in 100 μL dH2O, store at -20 °C. Lyophilized primers are stable for up to one year when stored at ambient temperature. Following dissolution in 10 μL dH2O, store at -20 °C.
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Expiry Date
- 12 months
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: "STAT5-glucocorticoid receptor interaction and MTF-1 regulate the expression of ZnT2 (Slc30a2) in pancreatic acinar cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 107, Issue 7, pp. 2818-23, (2010) (PubMed).
: "Regulation of prion gene expression by transcription factors SP1 and metal transcription factor-1." in: The Journal of biological chemistry, Vol. 284, Issue 2, pp. 1291-301, (2009) (PubMed).
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: "STAT5-glucocorticoid receptor interaction and MTF-1 regulate the expression of ZnT2 (Slc30a2) in pancreatic acinar cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 107, Issue 7, pp. 2818-23, (2010) (PubMed).
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- MTF1 (Metal-Regulatory Transcription Factor 1 (MTF1))
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Alternative Name
- MTF1
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Background
- This gene encodes a transcription factor that induces expression of metallothioneins and other genes involved in metal homeostasis in response to heavy metals such as cadmium, zinc, copper, and silver. The protein is a nucleocytoplasmic shuttling protein that accumulates in the nucleus upon heavy metal exposure and binds to promoters containing a metal-responsive element (MRE). [provided by RefSeq, Jul 2008].
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NCBI Accession
- NM_005955, NP_005946
Target
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