Human MYB cDNA Clone in Mammalian Expression Vector
This is a V-Myb Myeloblastosis Viral Oncogene Homolog (Avian) plasmid from OriGene - 2 times cited - with cDNA insert cloned into Mammalian Expression VectorpCMV6-XL5. Insert length: 3180 bp. Transient expression. Suitable for PExp.
Bacterial selection: Ampicillin.
1 image
(2 references)
Catalog No. ABIN3385310
Supplier Product No.: sc116779
$886.05
Plus shipping costs $50.00
10 μg
Shipping to:
United States
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Quick Overview for Human MYB cDNA Clone in Mammalian Expression Vector (ABIN3385310)
Gene
MYB
(V-Myb Myeloblastosis Viral Oncogene Homolog (Avian) (MYB))
Application
Protein Expression (PExp)
Insert
cDNA
Vector
Mammalian Expression Vector
Vector Backbone
pCMV6-XL5
Promoter
Enhanced CMV Promoter, T7 Promoter
Bacterial Resistance
Ampicillin
Expression Type
Transient
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Species
- Human
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Supplier Product No.
- sc116779
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Supplier
- OriGene
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Purpose
- Untagged full-length cDNA clone from Human MYB is ideal for over-expression of native protein for functional studies.
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Specificity
- Restriction Site: NotI-NotI
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Characteristics
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- These cDNA clones are isolated from full-length cDNA libraries and usually contain the coding sequence as well as the untranslated regions (UTRs) of the mRNA transcript appropriate to the library from which they were isolated.
- These cDNA clones are ideal for over-expression of native proteins for functional studies. Provided as 10 μg transfection-ready plasmids.
- Every lot of primer is tested to provide clean sequencing of cDNA clones.
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Purification
- The DNAs were purified using PowerPrep HP Plasmid isolation kits for transfection ready plasmids.
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Components
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- The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA.
- The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
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Insert Length
- 3180 bp
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Sequencing Primer
- VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3', XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Storage
- RT,-20 °C
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Storage Comment
- The lyophilized plasmid is stable for up to one year when stored at ambient temperature. Following dissolution in 100 μL dH2O, store at -20 °C. Lyophilized primers are stable for up to one year when stored at ambient temperature. Following dissolution in 10 μL dH2O, store at -20 °C.
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Expiry Date
- 12 months
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: "Myb overexpression overrides androgen depletion-induced cell cycle arrest and apoptosis in prostate cancer cells, and confers aggressive malignant traits: potential role in castration resistance." in: Carcinogenesis, Vol. 33, Issue 6, pp. 1149-57, (2012) (PubMed).
: "c-myb activates CXCL12 transcription in T47D and MCF7 breast cancer cells." in: Acta biochimica et biophysica Sinica, Vol. 42, Issue 1, pp. 1-7, (2009) (PubMed).
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: "Myb overexpression overrides androgen depletion-induced cell cycle arrest and apoptosis in prostate cancer cells, and confers aggressive malignant traits: potential role in castration resistance." in: Carcinogenesis, Vol. 33, Issue 6, pp. 1149-57, (2012) (PubMed).
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- MYB (V-Myb Myeloblastosis Viral Oncogene Homolog (Avian) (MYB))
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Alternative Name
- MYB
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Background
- This gene encodes a protein with three HTH DNA-binding domains that functions as a transcription regulator. This protein plays an essential role in the regulation of hematopoiesis. This gene may be aberrently expressed or rearranged or undergo translocation in leukemias and lymphomas, and is considered to be an oncogene. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2016].Transcript Variant: This variant (2) lacks an alternate in-frame exon in the coding region, as compared to variant 1. The resulting isoform (2) is shorter than isoform 1.
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NCBI Accession
- NM_005375, NP_005366
Target
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