Human NOX4 cDNA Clone in Mammalian Expression Vector
This is a NADPH Oxidase 4 plasmid from OriGene - 2 times cited - with cDNA insert cloned into Mammalian Expression VectorpCMV6-XL5. Insert length: 2100 bp. Transient expression. Suitable for PExp.
Bacterial selection: Ampicillin.
1 image
(2 references)
Catalog No. ABIN3385488
Supplier Product No.: sc310253
$799.92
Plus shipping costs $50.00
10 μg
Shipping to:
United States
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Quick Overview for Human NOX4 cDNA Clone in Mammalian Expression Vector (ABIN3385488)
Gene
NADPH Oxidase 4 (NOX4)
Application
Protein Expression (PExp)
Insert
cDNA
Vector
Mammalian Expression Vector
Vector Backbone
pCMV6-XL5
Promoter
Enhanced CMV Promoter, T7 Promoter
Bacterial Resistance
Ampicillin
Expression Type
Transient
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Species
- Human
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Supplier Product No.
- sc310253
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Supplier
- OriGene
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Purpose
- Untagged full-length cDNA clone from Human NOX4 is ideal for over-expression of native protein for functional studies.
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Specificity
- Restriction Site: NotI-NotI
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Characteristics
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- These cDNA clones are isolated from full-length cDNA libraries and usually contain the coding sequence as well as the untranslated regions (UTRs) of the mRNA transcript appropriate to the library from which they were isolated.
- These cDNA clones are ideal for over-expression of native proteins for functional studies. Provided as 10 μg transfection-ready plasmids.
- Every lot of primer is tested to provide clean sequencing of cDNA clones.
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Purification
- The DNAs were purified using PowerPrep HP Plasmid isolation kits for transfection ready plasmids.
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Components
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- The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA.
- The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
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Insert Length
- 2100 bp
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Sequencing Primer
- VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3', XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Storage
- RT,-20 °C
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Storage Comment
- The lyophilized plasmid is stable for up to one year when stored at ambient temperature. Following dissolution in 100 μL dH2O, store at -20 °C. Lyophilized primers are stable for up to one year when stored at ambient temperature. Following dissolution in 10 μL dH2O, store at -20 °C.
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Expiry Date
- 12 months
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: "NADPH oxidase 4 induces cardiac arrhythmic phenotype in zebrafish." in: The Journal of biological chemistry, Vol. 289, Issue 33, pp. 23200-8, (2014) (PubMed).
: "Netrin-1 abrogates ischemia/reperfusion-induced cardiac mitochondrial dysfunction via nitric oxide-dependent attenuation of NOX4 activation and recoupling of NOS." in: Journal of molecular and cellular cardiology, Vol. 78, pp. 174-85, (2014) (PubMed).
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: "NADPH oxidase 4 induces cardiac arrhythmic phenotype in zebrafish." in: The Journal of biological chemistry, Vol. 289, Issue 33, pp. 23200-8, (2014) (PubMed).
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- NADPH Oxidase 4 (NOX4)
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Alternative Name
- NOX4
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Background
- This gene encodes a member of the NOX-family of enzymes that functions as the catalytic subunit the NADPH oxidase complex. The encoded protein is localized to non-phagocytic cells where it acts as an oxygen sensor and catalyzes the reduction of molecular oxygen to various reactive oxygen species (ROS). The ROS generated by this protein have been implicated in numerous biological functions including signal transduction, cell differentiation and tumor cell growth. A pseudogene has been identified on the other arm of chromosome 11. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Jan 2009].Transcript Variant: This variant (1) encodes isoform (a).
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NCBI Accession
- NM_016931, NP_058627
Target
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