Human PARK7 cDNA Clone in Mammalian Expression Vector
This is a Parkinson Protein 7 plasmid from OriGene - 3 times cited - with cDNA insert cloned into Mammalian Expression VectorpCMV6-XL5. Insert length: 1000 bp. Transient expression. Suitable for PExp.
Bacterial selection: Ampicillin.
1 image
(3 references)
Catalog No. ABIN3385676
Supplier Product No.: sc115623
$297.00
Plus shipping costs $50.00
10 μg
Shipping to:
United States
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Quick Overview for Human PARK7 cDNA Clone in Mammalian Expression Vector (ABIN3385676)
Gene
PARK7/DJ1 (PARK7)
(Parkinson Protein 7 (PARK7))
Application
Protein Expression (PExp)
Insert
cDNA
Vector
Mammalian Expression Vector
Vector Backbone
pCMV6-XL5
Promoter
Enhanced CMV Promoter, T7 Promoter
Bacterial Resistance
Ampicillin
Expression Type
Transient
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Species
- Human
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Supplier Product No.
- sc115623
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Supplier
- OriGene
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Purpose
- Untagged full-length cDNA clone from Human PARK7 is ideal for over-expression of native protein for functional studies.
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Specificity
- Restriction Site: NotI-NotI
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Characteristics
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- These cDNA clones are isolated from full-length cDNA libraries and usually contain the coding sequence as well as the untranslated regions (UTRs) of the mRNA transcript appropriate to the library from which they were isolated.
- These cDNA clones are ideal for over-expression of native proteins for functional studies. Provided as 10 μg transfection-ready plasmids.
- Every lot of primer is tested to provide clean sequencing of cDNA clones.
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Purification
- The DNAs were purified using PowerPrep HP Plasmid isolation kits for transfection ready plasmids.
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Components
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- The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA.
- The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
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Insert Length
- 1000 bp
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Sequencing Primer
- VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3', XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Storage
- RT,-20 °C
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Storage Comment
- The lyophilized plasmid is stable for up to one year when stored at ambient temperature. Following dissolution in 100 μL dH2O, store at -20 °C. Lyophilized primers are stable for up to one year when stored at ambient temperature. Following dissolution in 10 μL dH2O, store at -20 °C.
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Expiry Date
- 12 months
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: "DJ-1 promotes invasion and metastasis of pancreatic cancer cells by activating SRC/ERK/uPA." in: Carcinogenesis, Vol. 33, Issue 3, pp. 555-62, (2012) (PubMed).
: "PINK1-dependent recruitment of Parkin to mitochondria in mitophagy." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 107, Issue 1, pp. 378-83, (2010) (PubMed).
: "The 1p-encoded protein stathmin and resistance of malignant gliomas to nitrosoureas." in: Journal of the National Cancer Institute, Vol. 99, Issue 8, pp. 639-52, (2007) (PubMed).
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- PARK7/DJ1 (PARK7) (Parkinson Protein 7 (PARK7))
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Alternative Name
- PARK7
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Background
- The product of this gene belongs to the peptidase C56 family of proteins. It acts as a positive regulator of androgen receptor-dependent transcription. It may also function as a redox-sensitive chaperone, as a sensor for oxidative stress, and it apparently protects neurons against oxidative stress and cell death. Defects in this gene are the cause of autosomal recessive early-onset Parkinson disease 7. Two transcript variants encoding the same protein have been identified for this gene. [provided by RefSeq, Jul 2008].Transcript Variant: This variant (1) represents the longer transcript. Both variants 1 and 2 encode the same protein.
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NCBI Accession
- NM_007262, NP_009193
Target
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