Human PDE4B cDNA Clone in Mammalian Expression Vector
This is a phosphodiesterase 4B, cAMP-Specific plasmid from OriGene with cDNA insert cloned into Mammalian Expression VectorpCMV6-XL5. Insert length: not_set. Transient expression. Suitable for PExp.
Bacterial selection: Ampicillin.
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Catalog No. ABIN3385726
Supplier Product No.: sc302893
$520.74
Plus shipping costs $50.00
10 μg
Shipping to:
United States
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Quick Overview for Human PDE4B cDNA Clone in Mammalian Expression Vector (ABIN3385726)
Gene
PDE4B
(phosphodiesterase 4B, cAMP-Specific (PDE4B))
Application
Protein Expression (PExp)
Insert
cDNA
Vector
Mammalian Expression Vector
Vector Backbone
pCMV6-XL5
Promoter
Enhanced CMV Promoter, T7 Promoter
Bacterial Resistance
Ampicillin
Expression Type
Transient
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Species
- Human
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Supplier Product No.
- sc302893
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Supplier
- OriGene
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Purpose
- Untagged full-length cDNA clone from Human PDE4B is ideal for over-expression of native protein for functional studies.
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Specificity
- Restriction Site: NotI-NotI
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Characteristics
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- These cDNA clones are isolated from full-length cDNA libraries and usually contain the coding sequence as well as the untranslated regions (UTRs) of the mRNA transcript appropriate to the library from which they were isolated.
- These cDNA clones are ideal for over-expression of native proteins for functional studies. Provided as 10 μg transfection-ready plasmids.
- Every lot of primer is tested to provide clean sequencing of cDNA clones.
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Purification
- The DNAs were purified using PowerPrep HP Plasmid isolation kits for transfection ready plasmids.
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Components
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- The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA.
- The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
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Sequencing Primer
- VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3', XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Storage
- RT,-20 °C
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Storage Comment
- The lyophilized plasmid is stable for up to one year when stored at ambient temperature. Following dissolution in 100 μL dH2O, store at -20 °C. Lyophilized primers are stable for up to one year when stored at ambient temperature. Following dissolution in 10 μL dH2O, store at -20 °C.
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Expiry Date
- 12 months
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- PDE4B (phosphodiesterase 4B, cAMP-Specific (PDE4B))
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Alternative Name
- PDE4B
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Background
- This gene is a member of the type IV, cyclic AMP (cAMP)-specific, cyclic nucleotide phosphodiesterase (PDE) family. The encoded protein regulates the cellular concentrations of cyclic nucleotides and thereby play a role in signal transduction. Altered activity of this protein has been associated with schizophrenia and bipolar affective disorder. Alternative splicing and the use of alternative promoters results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2014].Transcript Variant: This variant (b) differs in the 5' UTR and lacks a portion of the 5' coding region, compared to variant a. It represents use of an alternate promoter and initiates translation at an alternate start codon. The encoded isoform (2, also known as PDE4B2 or PDE32) has a shorter and distinct N-terminus when compared to isoform 1.
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NCBI Accession
- NM_001037339, NP_001032416
Target
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