Human PEA15 cDNA Clone in Mammalian Expression Vector
This is a phosphoprotein Enriched in Astrocytes 15 plasmid from OriGene - one-time cited - with cDNA insert cloned into Mammalian Expression VectorpCMV6-XL5. Insert length: 1700 bp. Transient expression. Suitable for PExp.
Bacterial selection: Ampicillin.
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Catalog No. ABIN3385749
Supplier Product No.: sc108132
$222.75
Plus shipping costs $50.00
10 μg
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Quick Overview for Human PEA15 cDNA Clone in Mammalian Expression Vector (ABIN3385749)
Gene
PEA15
(phosphoprotein Enriched in Astrocytes 15 (PEA15))
Application
Protein Expression (PExp)
Insert
cDNA
Vector
Mammalian Expression Vector
Vector Backbone
pCMV6-XL5
Promoter
Enhanced CMV Promoter, T7 Promoter
Bacterial Resistance
Ampicillin
Expression Type
Transient
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Species
- Human
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Supplier Product No.
- sc108132
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Supplier
- OriGene
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Purpose
- Untagged full-length cDNA clone from Human PEA15 is ideal for over-expression of native protein for functional studies.
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Specificity
- Restriction Site: NotI-NotI
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Characteristics
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- These cDNA clones are isolated from full-length cDNA libraries and usually contain the coding sequence as well as the untranslated regions (UTRs) of the mRNA transcript appropriate to the library from which they were isolated.
- These cDNA clones are ideal for over-expression of native proteins for functional studies. Provided as 10 μg transfection-ready plasmids.
- Every lot of primer is tested to provide clean sequencing of cDNA clones.
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Purification
- The DNAs were purified using PowerPrep HP Plasmid isolation kits for transfection ready plasmids.
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Components
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- The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA.
- The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
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Insert Length
- 1700 bp
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Sequencing Primer
- VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3', XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Storage
- RT,-20 °C
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Storage Comment
- The lyophilized plasmid is stable for up to one year when stored at ambient temperature. Following dissolution in 100 μL dH2O, store at -20 °C. Lyophilized primers are stable for up to one year when stored at ambient temperature. Following dissolution in 10 μL dH2O, store at -20 °C.
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Expiry Date
- 12 months
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: "PTEN loss promotes mitochondrially dependent type II Fas-induced apoptosis via PEA-15." in: Molecular and cellular biology, Vol. 29, Issue 5, pp. 1222-34, (2009) (PubMed).
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: "PTEN loss promotes mitochondrially dependent type II Fas-induced apoptosis via PEA-15." in: Molecular and cellular biology, Vol. 29, Issue 5, pp. 1222-34, (2009) (PubMed).
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- PEA15 (phosphoprotein Enriched in Astrocytes 15 (PEA15))
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Alternative Name
- PEA15
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Background
- This gene encodes a death effector domain-containing protein that functions as a negative regulator of apoptosis. The encoded protein is an endogenous substrate for protein kinase C. This protein is also overexpressed in type 2 diabetes mellitus, where it may contribute to insulin resistance in glucose uptake. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014].Transcript Variant: This variant (2) lacks two alternate exons in the 5' region and initiates translation at a downstream in-frame start codon, compared to variant 1. The encoded isoform (b) has a shorter N-terminus than isoform a. Variants 2 and 3 encode the same isoform (b).
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NCBI Accession
- NM_003768, NP_003759
Target
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